ID 109529
Title Alternative
歯原性上皮細胞におけるCtip2によるSp6遺伝子の転写制御
Role of Ctip2 in Sp6 regulation
Author
Adiningrat, Arya the University of Tokushima
Tanimura, Ayako the University of Tokushima KAKEN Search Researchers
Yanuaryska, Ryna Dwi the University of Tokushima
Hagita, Hiroko the University of Tokushima
Keywords
ameloblast differentiation
Ctip2
Sp6
tooth development
transcriptional regulation
Content Type
Thesis or Dissertation
Description
Tooth development relies on the interaction between the oral ectoderm and underlying mesenchyme, and is regulated by a complex genetic cascade. This transcriptional cascade is regulated by the spatiotemporal activation and deactivation of transcription factors.The specificity proteins 6 (Sp6) and chicken ovalbumin upstream promoter transcription factor-interacting protein 2 (Ctip2) were identified in loss-of-function studies as key transcription factors required for tooth development. Ctip2 binds to the Sp6 promoter in vivo ; however, its role in Sp6 expression remains unclear. In this study, we investigated Sp6 transcriptional regulation by Ctip2. Immunohistochemical analysis revealed that Sp6 and Ctip2 colocalize in the rat incisor during tooth development. We examined whether Ctip2 regulates Sp6 promoter activity in dental epithelial cells. Cotransfection experiments using serial Sp6 promoter-luciferase constructs and Ctip2 expression plasmids showed that Ctip2 significantly suppressed the Sp6 second promoter activity, although the Sp6 first promoter activity was unaffected. Ctip2 was able to bind to the proximal region of the Sp6 first promoter, as previously demonstrated, and also to the novel distal region of the first, and second promoter regions. Our findings indicate that Ctip2 regulates Sp6 gene expression through direct binding to the Sp6 second promoter region.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
61
Issue
1-2
Start Page
126
End Page
136
Sort Key
126
Published Date
2014-02
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨 : LID201509181010.pdf
審査要旨 : LID201509181011.pdf
論文本文 : LID201509181012.pdf
本論文は,著者Arya Adiningratの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第2859号
Diploma Number
甲口第398号
Granted Date
2015-09-16
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Oral Sciences