ID 109529
著者
Adiningrat, Arya Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School
谷村, 綾子 Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN研究者をさがす
三好, 圭子 Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
Yanuaryska, Ryna Dwi Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School
ハギタ, ヒロコ Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School
堀口, 大吾 Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
野間, 隆文 Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
ameloblast differentiation
Ctip2
Sp6
tooth development
transcriptional regulation
資料タイプ
学術雑誌論文
抄録
Tooth development relies on the interaction between the oral ectoderm and underlying
mesenchyme, and is regulated by a complex genetic cascade. This transcriptional
cascade is regulated by the spatiotemporal activation and deactivation of transcription factors.The specificity proteins 6 (Sp6) and chicken ovalbumin upstream promoter transcription factor-interacting protein 2 (Ctip2) were identified in loss-of-function studies as key transcription factors required for tooth development. Ctip2 binds to the Sp6 promoter in vivo ; however, its role in Sp6 expression remains unclear. In this study, we investigated Sp6 transcriptional regulation by Ctip2. Immunohistochemical analysis revealed that Sp6 and Ctip2 colocalize in the rat incisor during tooth development. We examined whether Ctip2 regulates Sp6 promoter activity in dental epithelial cells. Cotransfection experiments using serial Sp6 promoter-luciferase constructs and Ctip2 expression plasmids showed that Ctip2
significantly suppressed the Sp6 second promoter activity, although the Sp6 first promoter activity was unaffected. Ctip2 was able to bind to the proximal region of the Sp6 first promoter, as previously demonstrated, and also to the novel distal region of the first, and second promoter regions. Our findings indicate that Ctip2 regulates Sp6 gene expression through direct binding to the Sp6 second promoter region.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
61
1-2
開始ページ
126
終了ページ
136
並び順
126
発行日
2014-2
EDB ID
277120
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
歯学系