ID 109655
Author
Hossain, Md. Golam Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School
Iwata, Takeo Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School KAKEN Search Researchers
Mizusawa, Noriko Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Shima, Shahidan Wan Nazatul Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School
Okutsu, Toru Department of Oral and Maxillofacial Prosthodontics, Institute of Health Biosciences, The University of Tokushima Graduate School
Ishimoto, Kyoko Department of Orthodontics and Dentofacial Orthopedics, Institute of Health Biosciences, The University of Tokushima Graduate School KAKEN Search Researchers
Yoshimoto, Katsuhiko Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
Compressive force
preadipocytes
adipogenesis
COX-2
PPARγ2
C/EBPα
Content Type
Journal Article
Description
Various mechanical stimuli affect differentiation of mesoderm-derived cells such as osteoblasts or myoblasts, suggesting that adipogenesis may be also influenced by mechanical stimulation. However, effects of mechanical stimuli on adipogenesis are
scarcely known. Compressive force was applied to a human preadipocyte cell line, SGBS. Levels of gene expression were estimated by real-time reverse transcription-polymerase chain reaction. The accumulation of lipids was evaluated by Sudan III or Oil Red O staining. In SGBS cells subjected to a compressive force of 226 Pa for 12 h before adipogenic induction, adipogenesis was inhibited. Compressive force immediately after adipogenic induction did not affect the adipogenesis. The expression of peroxisome proliferator-activated receptor (PPAR) γ2 and CCAAT/enhancer binding protein (C/EBP) α mRNA during adipogenesis was inhibited by compressive force, whereas C/EBPβ and C/EBPδ mRNA levels were unaffected. In preadipocytes, compressive force increased mRNA levels of Krüppel-like factor 2, preadipocyte factor 1, WNT10b, and cyclooxygenase-2 (COX-2) which are known as negative regulators for the PPARγ2 and C/EBPα genes. Furthermore, a COX-2 inhibitor completely reversed the inhibition of adipogenesis by compressive force. In conclusion, compressive force inhibited adipogenesis by suppressing expression of PPARγ2 and C/EBPα in a COX-2-dependent manner.
Journal Title
Journal of Bioscience and Bioengineering
ISSN
13891723
NCID
AA11307678
Volume
109
Issue
3
Start Page
297
End Page
303
Sort Key
297
Published Date
2010-03
Remark
© 2009, The Society for Biotechnology, Japan. All rights reserved.
© 2009. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
Published Source
Journal of Bioscience and Bioengineering (2010) Volume 109 Issue 3 P.297–303 (doi:10.1016/j.jbiosc.2009.09.003)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Oral Sciences
University Hospital