アクセス数 : ?
ダウンロード数 : ?
ID 109655
著者
Hossain, Md. Golam Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School
岩田, 武男 Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School KAKEN研究者をさがす
水澤, 典子 Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
Shima, Shahidan Wan Nazatul Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School
オクツ, トオル Department of Oral and Maxillofacial Prosthodontics, Institute of Health Biosciences, The University of Tokushima Graduate School
石本, 恭子 Department of Orthodontics and Dentofacial Orthopedics, Institute of Health Biosciences, The University of Tokushima Graduate School KAKEN研究者をさがす
吉本, 勝彦 Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
Compressive force
preadipocytes
adipogenesis
COX-2
PPARγ2
C/EBPα
資料タイプ
学術雑誌論文
抄録
Various mechanical stimuli affect differentiation of mesoderm-derived cells such as osteoblasts or myoblasts, suggesting that adipogenesis may be also influenced by mechanical stimulation. However, effects of mechanical stimuli on adipogenesis are
scarcely known. Compressive force was applied to a human preadipocyte cell line, SGBS. Levels of gene expression were estimated by real-time reverse transcription-polymerase chain reaction. The accumulation of lipids was evaluated by Sudan III or Oil Red O staining. In SGBS cells subjected to a compressive force of 226 Pa for 12 h before adipogenic induction, adipogenesis was inhibited. Compressive force immediately after adipogenic induction did not affect the adipogenesis. The expression of peroxisome proliferator-activated receptor (PPAR) γ2 and CCAAT/enhancer binding protein (C/EBP) α mRNA during adipogenesis was inhibited by compressive force, whereas C/EBPβ and C/EBPδ mRNA levels were unaffected. In preadipocytes, compressive force increased mRNA levels of Krüppel-like factor 2, preadipocyte factor 1, WNT10b, and cyclooxygenase-2 (COX-2) which are known as negative regulators for the PPARγ2 and C/EBPα genes. Furthermore, a COX-2 inhibitor completely reversed the inhibition of adipogenesis by compressive force. In conclusion, compressive force inhibited adipogenesis by suppressing expression of PPARγ2 and C/EBPα in a COX-2-dependent manner.
掲載誌名
Journal of Bioscience and Bioengineering
ISSN
13891723
cat書誌ID
AA11307678
109
3
開始ページ
297
終了ページ
303
並び順
297
発行日
2010-03
備考
© 2009, The Society for Biotechnology, Japan. All rights reserved.
© 2009. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
出典
Journal of Bioscience and Bioengineering (2010) Volume 109 Issue 3 P.297–303 (doi:10.1016/j.jbiosc.2009.09.003)
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
歯学系
病院