ID 109781
Title Transcription
マスピン ワ センザイガタ TGF-β ノ チクセキ オ カイシテ コツキシツ ノ セイジュク ニ カンヨ シテ イル
Title Alternative
Maspin is Involved in Bone Matrix Maturation by Enhancing the Accumulation of Latent TGF-β
Author
Tokuyama, Reiko Department of Oral and Maxillofacial Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School
Keywords
マスピン
TGF-β
細胞外基質
骨形成
セリンプロテアーゼ
Content Type
Journal Article
Description
Introduction: Maspin is a serine protease inhibitor that exhibits tumor suppressive and anti-angiogenic activities. This study was performed to elucidate a possible role for maspin in bone formation.
Materials and Methods: We performed immunohistochemical analysis of the expression of maspin during endochondral ossification. We evaluated the expression of maspin mRNA and protein in ROS 17/2.8 cells and primary rat osteoblastic cells by RT-PCR, immunocytochemistry and Western blot analysis. We also examined the accumulation of TGF-β in the extracellular matrix of cultured ROS 17/2.8 cells following transfection with vectors expressing either maspin or maspin antisense.
Results: We observed expression of maspin by active osteoblasts in vivo. Rat osteoblastic cells also expressed maspin mRNA and protein in vitro. Moreover, the accumulation of latent TGF-β in the extracellular matrix significantly decreased in cultures exposed to an anti-maspin antibody and when cells were transfected with a maspin antisense-expressing vector. In contrast, accumulation of latent TGF-β in the extracellular matrix increased following transfection of cells with a vector expressing maspin.
Conclusions: These findings suggest that maspin expressed in active osteoblasts plays an important physiological role during maturation of the bone matrix, and in particular during the process of accumulation of latent TGF-β in the extracellular matrix.
Journal Title
四国歯学会雑誌
ISSN
09146091
NCID
AN10050046
Publisher
四国歯学会
Volume
21
Issue
2
Start Page
341
End Page
348
Sort Key
341
Published Date
2009-01-31
FullText File
language
jpn
TextVersion
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