ID 109781
タイトルヨミ
マスピン ワ センザイガタ TGF-β ノ チクセキ オ カイシテ コツキシツ ノ セイジュク ニ カンヨ シテ イル
タイトル別表記
Maspin is Involved in Bone Matrix Maturation by Enhancing the Accumulation of Latent TGF-β
著者
徳山, 麗子 徳島大学大学院ヘルスバイオサイエンス研究部顎口腔再建医学講座口腔顎顔面外科学分野
キーワード
マスピン
TGF-β
細胞外基質
骨形成
セリンプロテアーゼ
資料タイプ
学術雑誌論文
抄録
Introduction: Maspin is a serine protease inhibitor that exhibits tumor suppressive and anti-angiogenic activities. This study was performed to elucidate a possible role for maspin in bone formation.
Materials and Methods: We performed immunohistochemical analysis of the expression of maspin during endochondral ossification. We evaluated the expression of maspin mRNA and protein in ROS 17/2.8 cells and primary rat osteoblastic cells by RT-PCR, immunocytochemistry and Western blot analysis. We also examined the accumulation of TGF-β in the extracellular matrix of cultured ROS 17/2.8 cells following transfection with vectors expressing either maspin or maspin antisense.
Results: We observed expression of maspin by active osteoblasts in vivo. Rat osteoblastic cells also expressed maspin mRNA and protein in vitro. Moreover, the accumulation of latent TGF-β in the extracellular matrix significantly decreased in cultures exposed to an anti-maspin antibody and when cells were transfected with a maspin antisense-expressing vector. In contrast, accumulation of latent TGF-β in the extracellular matrix increased following transfection of cells with a vector expressing maspin.
Conclusions: These findings suggest that maspin expressed in active osteoblasts plays an important physiological role during maturation of the bone matrix, and in particular during the process of accumulation of latent TGF-β in the extracellular matrix.
掲載誌名
四国歯学会雑誌
ISSN
09146091
cat書誌ID
AN10050046
出版者
四国歯学会
21
2
開始ページ
341
終了ページ
348
並び順
341
発行日
2009-01-31
フルテキストファイル
言語
jpn
著者版フラグ
出版社版