細胞賦活効果を狙ったチタン表面処理法

Thromboembolism events around the cannula and cardiac periventricular were oftenly reported after implanting ventricular assist devices. To assist preventing these problems, we have studied the surface structural modification, based on the titanium (Ti) micro-porous structure, the chemical modification, and the hydro-oxidization treatment. The purpose of this study is the fabrication and evaluation of their fundamental modification that would be the factor of the neointimal growth in vivo.
Titanium powder was mixed with melted thermoplastic wax as the plastic compound method, and with calcium phosphate paste as the ceramics compound method. From each paste, adequate specimens were manufactured after sintering. Specimens were measured the porosity and the pore size, and were also evaluated in several mechanical properties. Compared to two methods for manufacturing the Ti micro-porous materials, the plastic compounds showed the fine characteristics in mechanical properties. But for applying as the scaffold for neointimal and blood compatible materials, these specimens would be better for promoting the blood vessel neointima.
For the chemical modification of Ti materials surface, bulk Ti specimens were operated with the hydroxide treatment for modification. For promoting the cell growth, type I collagen were put on as the amino group rich container, and fixed with isocyanate for deriving the urethane bonding on the surface. After these treatments, specimens were measured their fixed collagen. Specimens were also observed by SEM, and analyzed by X-ray photoelectron spectroscopy. Fibroblast cells were also harvested on specimens’ surface for studying the performances as the scaffold. Our hydroxide treatment showed the favorable performance for the stability of hydroxyl gel surface and modified collagen, and also indicated as the fine scaffold.
Our results indicated the possibility of application for perivascular position and blood contacting situation, by promoting the cell-activation depending on micro-porous structure and modified titanium surface. Especially by choosing and trying the adequate cell activating factor concerning among the multiple organs and implantable devices, these treatment would be widely applicable for them.