ID 110170
Author
Mizuno, T. Division of Energy System, Institute of Technology and Science, Tokushima University
Nakao, S. Division of Energy System, Institute of Technology and Science, Tokushima University
Mizutani, Y. Division of Energy System, Institute of Technology and Science, Tokushima University
Iwata T. Division of Energy System, Institute of Technology and Science, Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Content Type
Journal Article
Description
We have constructed an improved version of a photon-counting phase-modulation fluorometer (PC-PMF) with a maximum modulation frequency of 1.0 GHz, where a phase domain measurement is conducted with a time-correlated single-photon-counting electronics. While the basic concept of the PC-PMF has been reported previously by one of the authors, little attention has been paid to its significance, other than its weak fluorescence measurement capability. Recently, we have recognized the importance of the PC-PMF and its potential for fluorescence lifetime measurements. One important aspect of the PC-PMF is that it enables us to perform high-speed measurements that exceed the frequency bandwidths of the photomultiplier tubes that are commonly used as fluorescence detectors. We describe the advantages of the PC-PMF and demonstrate its usefulness based on fundamental performance tests. In our new version of the PC-PMF, we have used a laser diode (LD) as an excitation light source rather than the light-emitting diode that was used in the primary version. We have also designed a simple and stable LD driver to modulate the device. Additionally, we have obtained a sinusoidal histogram waveform that has multiple cycles within a time span to be measured, which is indispensable for precise phase measurements. With focus on the fluorescence intensity and the resolution time, we have compared the performance of the PC-PMF with that of a conventional PMF using the analogue light detection method.
Journal Title
Review of Scientific Instruments
ISSN
00346748
NCID
AA00817730
AA12442307
Volume
86
Issue
4
Start Page
043110
Sort Key
43110
Published Date
2015-04
Remark
Copyright © 2015 AIP Publishing LLC.
EDB ID
Published Source
Review of Scientific Instruments (2015) Vol.86 Issue 4 043110
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Science and Technology