ID 110170
著者
水野, 孝彦 Division of Energy System, Institute of Technology and Science, Tokushima University
ナカオ, セイジ Division of Energy System, Institute of Technology and Science, Tokushima University
水谷, 康弘 Division of Energy System, Institute of Technology and Science, Tokushima University
岩田, 哲郎 Division of Energy System, Institute of Technology and Science, Tokushima University 徳島大学 教育研究者総覧 KAKEN研究者をさがす
資料タイプ
学術雑誌論文
抄録
We have constructed an improved version of a photon-counting phase-modulation fluorometer (PC-PMF) with a maximum modulation frequency of 1.0 GHz, where a phase domain measurement is conducted with a time-correlated single-photon-counting electronics. While the basic concept of the PC-PMF has been reported previously by one of the authors, little attention has been paid to its significance, other than its weak fluorescence measurement capability. Recently, we have recognized the importance of the PC-PMF and its potential for fluorescence lifetime measurements. One important aspect of the PC-PMF is that it enables us to perform high-speed measurements that exceed the frequency bandwidths of the photomultiplier tubes that are commonly used as fluorescence detectors. We describe the advantages of the PC-PMF and demonstrate its usefulness based on fundamental performance tests. In our new version of the PC-PMF, we have used a laser diode (LD) as an excitation light source rather than the light-emitting diode that was used in the primary version. We have also designed a simple and stable LD driver to modulate the device. Additionally, we have obtained a sinusoidal histogram waveform that has multiple cycles within a time span to be measured, which is indispensable for precise phase measurements. With focus on the fluorescence intensity and the resolution time, we have compared the performance of the PC-PMF with that of a conventional PMF using the analogue light detection method.
掲載誌名
Review of Scientific Instruments
ISSN
00346748
cat書誌ID
AA00817730
AA12442307
86
4
開始ページ
043110
並び順
43110
発行日
2015-04
備考
Copyright © 2015 AIP Publishing LLC.
EDB ID
299382
出典
Review of Scientific Instruments (2015) Vol.86 Issue 4 043110
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
理工学系