ID 110172
Author
Ishikawa, Mizuki Graduate School of Integrated Arts and Sciences, Tokushima University
Muraguchi, Ryosuke Graduate School of Integrated Arts and Sciences, Tokushima University
Azuma, Ayako Graduate School of Integrated Arts and Sciences, Tokushima University
Nawata, Shogo Graduate School of Integrated Arts and Sciences, Tokushima University
Miya, Mutsumi Graduate School of Integrated Arts and Sciences, Tokushima University
Katsuura, Tetsuya Graduate School of Integrated Arts and Sciences, Tokushima University
Naito, Tohru Graduate School of Integrated Arts and Sciences, Tokushima University
Oyama, Yasuo Graduate School of Integrated Arts and Sciences, Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Content Type
Journal Article
Description
2,2-Dibromo-3-nitrilopropionamide (DBNPA) is a major biocide in hydraulic fracturing fluids. Most biocides in fracturing fluids are considered to have low acute toxicity to mammals, but little information is available in the literature regarding the toxic actions of DBNPA on mammalian cells. This information is important to suggest the DBNPA toxicity on wild mammals. In this study, the effects of DBNPA on rat thymocytes were studied using flow cytometric techniques in order to further characterize the cytotoxicity of DBNPA for its safe use. DBNPA at 3-7.5 μM produced a steep concentration-dependent increase in cell lethality. At 5 μM, DBNPA significantly depolarized membranes with disturbance of asymmetrical distribution of membrane phospholipids. The lethal effect of DBNPA was completely abolished under cold conditions, and was augmented in the presence of ethanol. It is suggested that the lethal action of DBNPA is linked to changes in membrane fluidity. Because the concentration-dependent change of DBNPA-induced lethal action was very steep under in vitro conditions, the adverse actions of DBNPA on wild mammals are concerning, even though such reports have not yet surfaced.
Journal Title
Toxicology Research
ISSN
20454538
Volume
2016
Issue
5
Start Page
1329
End Page
1334
Sort Key
1329
Published Date
2016-06-21
Remark
This journal is Copyright © The Royal Society of Chemistry 2016
EDB ID
Published Source
Toxicology Research (2016) Issue 5 p.1329-1334
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Bioscience and Bioindustry