ID 110702
Author
Sun, Xue-Zhi Environmental and Toxicological Sciences Research Group, National Institute of Radiological Sciences
Zhang, Rui Department of Anatomy and Developmental Neurobiology, The University of Tokushima School of Medicine
Cui, Chun Department of Anatomy and Developmental Neurobiology, The University of Tokushima School of Medicine
Takahashi, Sentaro Environmental and Toxicological Sciences Research Group, National Institute of Radiological Sciences
Kubota, Yoshihisa Environmental and Toxicological Sciences Research Group, National Institute of Radiological Sciences
Sawada, Kazuhiko Department of Anatomy and Developmental Neurobiology, The University of Tokushima School of Medicine
Fukui, Yoshihiro Department of Anatomy and Developmental Neurobiology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
cell adhesion molecular
irradiation
migration
neocortex
Content Type
Journal Article
Description
To gain insight to the cellular and molecular mechanisms involved abnormal neuronal migration induced by irradiation, we investigated expression of neuronal cell adhesion molecule L 1 and neuronal migration in the brains through comparison between rats prenatally exposed to X-ray and controls. To observe the pattern of neuronal migration, bromodeoxyuridine (BrdU) was chosen as a marker to label migrating cells. The results showed some of the labeled cells remained in the lower of the cortical plate in the irradiated rats, suggesting that neuronal migration was disrupted by X-ray. To study change of expressing neural cell molecule L1, rat brains were analyzed by SDS-PAGE after isolation of L1 by immunoaffinity chromatography. In the all brain membrane fraction, immunoaffinity purified L1 had bands at 200, 180, 140 and 80 kDa. However, the bands in the irradiated group were very weak when compared with the control. Taking these results into account, abnormal neuronal migration and reduction of expression L1 found in their radiated brain indicated that migration of neural cells may be largely dependent on radial glial fiber as well as neural cell molecules like L1. A decrease in L1 expressionmay be one of reasons of abnormal neuronal migration.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
50
Issue
3-4
Start Page
187
End Page
191
Sort Key
187
Published Date
2003
EDB ID
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences