ID 110758
Author
Bunpo, Piyawan Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School|Department of Biochemistry, Faculty of Medicine, Chiang Mai University
Kataoka, Keiko Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Arimochi, Hideki Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Nakayama, Haruyuki Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
Kuwahara, Tomomi Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
Vinitketkumnuen, Usanee Department of Biochemistry, Faculty of Medicine, Chiang Mai University
Ohnishi, Yoshinari Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory
Keywords
asiatic acid
apoptosis
CPT-11
combination
cytotoxicity
Content Type
Journal Article
Description
Asiatic acid is a pentacyclic triterpene contained inmedicinal plants. The cytotoxic effect of this compound and its augmentative effect on the anticancer drug irinotecan hydrochloride (CPT-11) were investigated in the human colon adenocarcinoma cell lineHT-29. Asiatic acid dose-dependently showed cytotoxicity in HT-29 cells. DNA fragmentation, annexin-positive apoptotic cells, andcaspase-3 activation were observed in a dose-dependent manner. Acaspase-3 inhibitor suppressed the DNA ladder formation in a concentration-dependent manner. Bcl-2 and Bcl-xL proteins were decreased by asiatic acid treatment. These results indicate that asiatic acid induced apoptosis inHT-29 cells viacaspase-3activation.Cytotoxic effectsof combined treatment with CPT-11 and asiatic acid on HT-29 cells were further examined. Simultaneous treatment or sequential exposure first to asiatic acid and then to CPT-11 showed an additive effect. Synergism was observed when cells were first exposed to CPT-11 and then to asiatic acid. These results suggest that asiatic acid can be used as an agent for increasing sensitivity of colon cancer cells to treatment with CPT-11 or as an agent for reducing adverse effects of CPT-11.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
52
Issue
1-2
Start Page
65
End Page
73
Sort Key
65
Published Date
2005-02
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences