ID 110796
Author
Kinoshita, Keigo Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School|Department of Digestive and Cardiovascular Medicine, Institute of Health Biosciences, The University of Tokushima Graduate School
Shinka, Toshikatsu Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School
Sato, Youichi Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kurahashi, Hiroki Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University
Kowa, Hiroe Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University
Chen, Gang Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School
Umeno, Mayumi Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School
Toida, Kazunori Department of Anatomy and Cell Biology, Institute of Health Biosciences, The University of Tokushima Graduate School
Kiyokage, Emi Department of Anatomy and Cell Biology, Institute of Health Biosciences, The University of Tokushima Graduate School
Nakano, Takuro Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School
Ito, Susumu Department of Digestive and Cardiovascular Medicine, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory
Nakahori, Yutaka Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School
Keywords
HSFY
mHSFYL
HSF
Y chromosome
spermatogenesis
Content Type
Journal Article
Description
Heat shock transcription factor on Y (HSFY) is located in one of three candidate regions for azoospermic factor (AZF), AZFb on the Y chromosome. We and others have already revealed that some azoospermic males are missing the regions of the Y chromosome including HSFY. Previously, we showed that murine HSFY-like sequence〔mHSFYL(RikencDNA4933413G11Rik)〕, which is the mouse orthologue of HSFY, is exclusively expressed in testis. The sequences encoding the presumed DNA-binding domain in HSFY and mHSFYL were found in other mammals such as dogs, cows and chickens. To elucidate mHSFYL expression in the testes in detail, we carried out in situ hybridization. mHSFYL was predominantly expressed in round spermatids. Furthermore, we clarified the intracellular distribution of mHSFYL inCOS1 cells with HA- or GFP-tagged proteins. Both HA-mHSFYL and GFP-mHSFYL were located in the nucleus. Our results suggest that HSFY/mHSFYL may have evolutionarily conserved functions for spermatogenesis.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
53
Issue
1-2
Start Page
117
End Page
122
Sort Key
117
Published Date
2006-02
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Pharmaceutical Sciences
Medical Sciences