Expression analysis of a mouse orthologue of HSFY, a candidate for the azoospermic factor on the human Y chromosome

Kinoshita, Keigo; Shinka, Toshikatsu; Sato, Youichi; Kurahashi, Hiroki; Kowa, Hiroe; Chen, Gang; Umeno, Mayumi; Toida, Kazunori; Kiyokage, Emi; Nakano, Takuro; Ito, Susumu; Nakahori, Yutaka

doi:10.2152/jmi.53.117

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Use this link to cite this item : https://repo.lib.tokushima-u.ac.jp/110796

ID	110796
Author	Kinoshita, Keigo Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School\|Department of Digestive and Cardiovascular Medicine, Institute of Health Biosciences, The University of Tokushima Graduate School Shinka, Toshikatsu Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School Sato, Youichi Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers Kurahashi, Hiroki Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University Kowa, Hiroe Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University Chen, Gang Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School Umeno, Mayumi Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School Toida, Kazunori Department of Anatomy and Cell Biology, Institute of Health Biosciences, The University of Tokushima Graduate School Kiyokage, Emi Department of Anatomy and Cell Biology, Institute of Health Biosciences, The University of Tokushima Graduate School Nakano, Takuro Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School Ito, Susumu Department of Digestive and Cardiovascular Medicine, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory Nakahori, Yutaka Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School
Keywords	HSFY mHSFYL HSF Y chromosome spermatogenesis
Content Type	Journal Article
Description	Heat shock transcription factor on Y (HSFY) is located in one of three candidate regions for azoospermic factor (AZF), AZFb on the Y chromosome. We and others have already revealed that some azoospermic males are missing the regions of the Y chromosome including HSFY. Previously, we showed that murine HSFY-like sequence〔mHSFYL(RikencDNA4933413G11Rik)〕, which is the mouse orthologue of HSFY, is exclusively expressed in testis. The sequences encoding the presumed DNA-binding domain in HSFY and mHSFYL were found in other mammals such as dogs, cows and chickens. To elucidate mHSFYL expression in the testes in detail, we carried out in situ hybridization. mHSFYL was predominantly expressed in round spermatids. Furthermore, we clarified the intracellular distribution of mHSFYL inCOS1 cells with HA- or GFP-tagged proteins. Both HA-mHSFYL and GFP-mHSFYL were located in the nucleus. Our results suggest that HSFY/mHSFYL may have evolutionarily conserved functions for spermatogenesis.
Journal Title	The journal of medical investigation : JMI
ISSN	13431420
NCID	AA11166929
Volume	53
Issue	1-2
Start Page	117
End Page	122
Sort Key	117
Published Date	2006-02
EDB ID	160586
DOI (Published Version)	10.2152/jmi.53.117
URL ( Publisher's Version )	https://doi.org/10.2152/jmi.53.117
FullText File	jmi_53_1-2_117.pdf 263 KB
language	eng
TextVersion	Publisher
departments	Pharmaceutical Sciences Medical Sciences