ID 110826
Author
Ueno, Akemichi Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Graduate School
Yamashita, Kikuji Department of Oral and Maxillofacial Anatomy, Institute of Health Biosciences, The University of Tokushima Graduate School KAKEN Search Researchers
Miyoshi, Keiko Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Horiguchi, Taigo Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Ruspita, Intan Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Graduate School
Abe, Kaori Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Graduate School KAKEN Search Researchers
Noma, Takafumi Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
dental pulp cells
osteoblast
extracellular matrix
mineralization
Content Type
Journal Article
Description
Dental pulp cells have a capacity to differentiate into mineralization-inducing cells. To clarify the molecular mechanism, we established an in vitro mineralization-inducing system by rat clonal dental pulp cell line, RPC-C2A, and tried to purify a mineralizationinducing factor in conditioned medium (CM) from preosteoblastic MC3T3-E1 cells. The active factor was impermeable to an ultrafiltrating membrane, and sedimented by ultracentrifugation. The sedimented factor was found as a needle-like structure about 1.3 μm in average length as observed by transmission electron microscopy. The factor contained type I collagen, suggesting not a matrix vesicle, but a soluble matrix. The mineralizationinducing activity was also detected in CM from primary culture of rat calvaria (RC) cells. These results suggested that the soluble matrices from osteoblastic cells serve, at least in part, as differentiation-inducing agents.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
53
Issue
3-4
Start Page
297
End Page
302
Sort Key
297
Published Date
2006-08
EDB ID
FullText File
language
eng
TextVersion
Publisher
departments
Oral Sciences
University Hospital