ID 110860
Author
Jung, Eun Ju Departments of Dermatology, Institute of Health Biosciences, the University of Tokushima Graduate School
Kawai, Tomoko Department of Stress Science, Institute of Health Biosciences, the University of Tokushima Graduate School
Park, Hwan Ki Division of Enzyme Pathophysiology, The Institute for Enzyme Research, the University of Tokushima
Kubo, Yoshiaki Departments of Dermatology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Rokutan, Kazuhito Department of Stress Science, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Arase, Seiji Departments of Dermatology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
ultraviolet B irradiation
skin damage
peripheral blood cells
gene expression
marker genes
Content Type
Journal Article
Description
Ultraviolet B (UVB) is a serious irritant for the skin and increases a risk for skin cancer. To identify UVB-sensitive genes in peripheral blood, 11 healthy male volunteers were exposed to 0.3 J/cm2 of narrow-band (NB)-UVB, about half of minimal erythema dose (MED) in Japanese, and gene expression in blood was analyzed at 4 h, 24 h, 4 d and 7 d after the irradiation using microarray carrying oligonucleotide probes for 2,000 stressresponsive genes. RNA prepared before the irradiation was used as a reference control. Microarray analysis identified 21 genes as UVB-responsive genes with a peak at 24 h in 6 subjects, and real-time PCR validated the significant down-regulation of 9 (ABCB10, ATF1, ABCD3, TANK, FAS, SLC30A9, CHUK, CASP1, and ABCE1) out of the 21 genes in 11 subjects. Considering sensitive and characteristic features of 9 marker genes, they may be useful indicators for monitoring systemic response to UVB irradiation.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
55
Issue
3-4
Start Page
204
End Page
210
Sort Key
204
Published Date
2008-08
EDB ID
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences