ID 110866
Author
Tokunaga, Itsuo Department of Forensic Medicine, Institute of Health Biosciences, the University of Tokushima Graduate School
Ishigami, Akiko Department of Forensic Medicine, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kubo, Shin-ichi Department of Forensic Medicine, Faculty of Medicine, Fukuoka University
Gotohda, Takako Department of Forensic Medicine, Institute of Health Biosciences, the University of Tokushima Graduate School
Kitamura, Osamu Department of Forensic Medicine, Institute of Health Biosciences, the University of Tokushima Graduate School
Keywords
methamphetamine
neurotoxicity
peroxidative DNA damage
8-OH-dG
brain
Content Type
Journal Article
Description
In this study, we investigated methamphetamine (METH)- induced peroxidative DNA damage in various regions of the rat brain. We injected METH to rats following 2 protocols. For the single administration experiment (group I), 50 mg/kg (i.p.) of METH was administered to observe the acute influence of METH. For the repeated administration experiment (group II), 10 mg/kg/day (i.p.) of METH was injected for 5 days. Immunohistochemically, peroxidative damage DNA, 8-hydroxy-2’- deoxyguanosine (8-OH-dG) was observed, and in situ apoptosis was also observed. In group I, immunoreactivity of 8-OH-dG was only enhanced in neurons of the nucleus accumben of METH-treated rats. On in situ apoptosis detection, positive findings were also enhanced in all examined parts compared to those in the control, though there were no significant increases in 8-OH-dG-immunopositive neurons except in the nucleus accumben. In group II, the nucleus accumben also showed enhanced 8-OH-dG immunopositivity compared to that in the control. There was no significant difference in apoptosis between the control and METH groups. Based on our observations, it is considered that METH induces oxidative DNA damage in the brain, especially in the nucleus accumben. However, those DNA damage might be caused differently between acute and chronic administration.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
55
Issue
3-4
Start Page
241
End Page
245
Sort Key
241
Published Date
2008-08
EDB ID
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences