ID 110880
Author
Nagao, Tamiko Department of Virology, Institute of Health Biosciences, the University of Tokushima Graduate School
Hatcho, Kazuki Department of Virology, Institute of Health Biosciences, the University of Tokushima Graduate School
Doi, Naoya Department of Virology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory
Fujiwara, Sachi Department of Virology, Institute of Health Biosciences, the University of Tokushima Graduate School
Adachi, Akio Department of Virology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Nomaguchi, Masako Department of Virology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
HIV-1
Gag
CA
CypA,
TRIM5α
Content Type
Journal Article
Description
We previously generated a prototype monkey-tropic human immunodeficiency virus type 1 (HIV-1) designated NL-DT5R. This viral clone has a small region of simian immunodeficiency virus (SIV) within Gag capsid (CA) protein and also SIV Vif protein, but displays a poor growth phenotype in simian cells. To improve the growth potential of NL-DT5R, we have constructed a series of its gag variant viruses. Out of fourteen viral clones generated, five were infectious for simian HSC-F cells, and two of the infectious variants grew similarly with NL-DT5R. Taking their genome structures into consideration, our data here clearly show that a narrow CA region within the Gag protein, i.e., the domain around cyclophilin A (CypA)-binding loop, is critical for the growth ability of HIV-1 in simian cells.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
56
Issue
1-2
Start Page
21
End Page
25
Sort Key
21
Published Date
2009-02
EDB ID
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences