ID 111018
Author
Kamae, Risa Tokushima University
Nojima, Shoko Osaka City University
Akiyoshi, Kenji Tokushima University
Setsu, Shoki Tokushima University
Honda, Sari Osaka City University
Masuda, Toshiya Osaka City University
Keywords
hydroxyhydroquinone
by-product
coffee
cytotoxicity
lymphocytes
calcium
Content Type
Journal Article
Description
Hydroxyhydroquinone (HHQ) is generated during coffee bean roasting. A cup of coffee contains 0.1–1.7 mg of HHQ. The actions of HHQ on mammalian DNA were examined because HHQ is a metabolite of benzene, which causes leukemia. Currently, information on the cellular actions of HHQ is limited. We examined the effects of sublethal levels of HHQ on the concentration of intracellular Ca2+ in rat thymic lymphocytes by using a flow cytometric technique with fluorescent probes. HHQ at 10 μM or more significantly elevated intracellular Ca2+ levels by increasing the membrane permeability of divalent cations, resulting in hyperpolarization via the activation of Ca2+-dependent K+ channels. HHQ-induced changes in the intracellular Ca2+ concentration and membrane potential may affect the cell functions of lymphocytes. HHQ-reduced coffee may be preferable in order to avoid the possible adverse effects of HHQ.
Journal Title
Food and Chemical Toxicology
ISSN
02786915
NCID
AA10627174
AA11526376
Publisher
Elsevier
Volume
102
Start Page
39
End Page
45
Published Date
2017-01-30
Rights
© 2017 Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Bioscience and Bioindustry