ID 111037
Title Alternative
Intelligent Measurement and Control of Tissue Collagen for Bone Tissue Engineering
Author
Keywords
bone
collagen
osteoblasts
tissue engineering
second-harmonic-generation
Content Type
Departmental Bulletin Paper
Description
Second-harmonic-generation (SHG) microscopy is a new tool for observing the collagen fiber in tissue in vivo. However, conventional SHG microscopy equipped with 100-fs pulse laser is insufficient to visualize collagen fiber produced by cultured cell with high contrast because of low nonlinear susceptibility x(2) in low-order-structured, immature collagen. To enhance the image contrast while avoiding the sample damage, one has to increase a peak power of the laser light while maintaining the same average power as the 100-fs laser. In this paper, we enhanced the sensitive of SHG microscopy by use of a 10-fs Ti:Sapphire laser and succeeded to visualize collagen fibers produced by the cultured osteoblasts in vivo.
Journal Title
Bulletin of Institute of Technology and Science
ISSN
21859094
NCID
AA12214889
Volume
60
Start Page
16
End Page
20
Sort Key
16
Published Date
2015
Rights
この論文の著作権は徳島大学ソシオテクノサイエンス研究部にあります。
FullText File
language
jpn
TextVersion
Publisher
departments
Science and Technology
University Hospital