Intelligent Measurement and Control of Tissue Collagen for Bone Tissue Engineering
Yasui, Takeshi Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Sato, Katsuya Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Departmental Bulletin Paper
Second-harmonic-generation (SHG) microscopy is a new tool for observing the collagen fiber in tissue in vivo. However, conventional SHG microscopy equipped with 100-fs pulse laser is insufficient to visualize collagen fiber produced by cultured cell with high contrast because of low nonlinear susceptibility x(2) in low-order-structured, immature collagen. To enhance the image contrast while avoiding the sample damage, one has to increase a peak power of the laser light while maintaining the same average power as the 100-fs laser. In this paper, we enhanced the sensitive of SHG microscopy by use of a 10-fs Ti:Sapphire laser and succeeded to visualize collagen fibers produced by the cultured osteoblasts in vivo.
Bulletin of Institute of Technology and Science
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