ID 111111
Author
Mitani, Tsuyoshi Tokushima University
Ibrahim Elhossany Elmarhomy, Ahmed Tokushima University
Dulamjav, Luvsandorj Tokushima University
Anu, Enkhtumur Tokushima University
Saito, Shohei Osaka University
Ishida, Shiro Tokushima Bunri University
Keywords
benzalkonium chloride
cytotoxicity
lymphocytes
zinc
Content Type
Journal Article
Description
Benzalkonium chloride (BZK) is a common preservative used in pharmaceutical and personal care products. ZnCl2 was recently reported to significantly potentiate the cytotoxicity of some biocidal compounds. In the present study, therefore, we compared the cytotoxic potency of BZK and then further studied the Zn2+-related actions of the most cytotoxic agent among BZK, using flow cytometric techniques with appropriate fluorescent probes in rat thymocytes. Cytotoxicity of benzylcetyldimethylammonium (BZK-C16) was more potent that those of benzyldodecyldimethylammonium and benzyldimethyltetradecylammonium. ZnCl2 (1–10 μM) significantly potentiated the cytotoxicity of BZK-C16 at a sublethal concentration (1 μM). The co-treatment of cells with 3 μM ZnCl2 and 1 μM BZK-C16 increased the population of both living cells with phosphatidylserine exposed on membrane surfaces and dead cells. BZK-C16 at 0.3–1.0 μM elevated intracellular Zn2+ levels by increasing Zn2+ influx, and augmented the cytotoxicity of 100 μM H2O2. Zn2+ is concluded to facilitate the toxicity of BZK. We suggest that the toxicity of BZK is determined after taking extracellular (plasma) and/or environmental Zn2+ levels into account.
Journal Title
Chemico-Biological Interactions
ISSN
00092797
NCID
AA0060252X
AA11523855
Publisher
Elsevier
Volume
268
Start Page
31
End Page
36
Published Date
2017-02-28
Rights
© 2017 Elsevier B.V. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Bioscience and Bioindustry