ID 111111
著者
Mitani, Tsuyoshi Tokushima University
Ibrahim Elhossany Elmarhomy, Ahmed Tokushima University
Dulamjav, Luvsandorj Tokushima University
Anu, Enkhtumur Tokushima University
Saito, Shohei Osaka University
Ishida, Shiro Tokushima Bunri University
キーワード
benzalkonium chloride
cytotoxicity
lymphocytes
zinc
資料タイプ
学術雑誌論文
抄録
Benzalkonium chloride (BZK) is a common preservative used in pharmaceutical and personal care products. ZnCl2 was recently reported to significantly potentiate the cytotoxicity of some biocidal compounds. In the present study, therefore, we compared the cytotoxic potency of BZK and then further studied the Zn2+-related actions of the most cytotoxic agent among BZK, using flow cytometric techniques with appropriate fluorescent probes in rat thymocytes. Cytotoxicity of benzylcetyldimethylammonium (BZK-C16) was more potent that those of benzyldodecyldimethylammonium and benzyldimethyltetradecylammonium. ZnCl2 (1–10 μM) significantly potentiated the cytotoxicity of BZK-C16 at a sublethal concentration (1 μM). The co-treatment of cells with 3 μM ZnCl2 and 1 μM BZK-C16 increased the population of both living cells with phosphatidylserine exposed on membrane surfaces and dead cells. BZK-C16 at 0.3–1.0 μM elevated intracellular Zn2+ levels by increasing Zn2+ influx, and augmented the cytotoxicity of 100 μM H2O2. Zn2+ is concluded to facilitate the toxicity of BZK. We suggest that the toxicity of BZK is determined after taking extracellular (plasma) and/or environmental Zn2+ levels into account.
掲載誌名
Chemico-Biological Interactions
ISSN
00092797
cat書誌ID
AA0060252X
AA11523855
出版者
Elsevier
268
開始ページ
31
終了ページ
36
発行日
2017-02-28
権利情報
© 2017 Elsevier B.V. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
322922
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
生物資源系