ID 111983
Title Alternative
Zinc is a determinant of the cytotoxicity of Ziram, a dithiocarbamate fungicide, in rat thymic lymphocytes : Possible environmental risk
Author
Kanemoto-Kataoka, Yumiko Tokushima University|Bayer Yakuhin, Ltd.
Oyama, Tomohiro M. Tokushima University|Medical Co. LTA
Ishibashi, Hitoshi Tokushima University|Kitasato University
Keywords
Fungicide
Dithiocarbamate
Lymphocytes
Cytotoxicity
Zinc
Content Type
Journal Article
Description
Ziram, one of dithiocarbamate fungicides, is widely applied to agriculture because the agent protects various crops from fungal infections. Risks of dithiocarbamate biocide use are concerned. It was previously reported that Ziram increased the intracellular concentration of Zn2+. Therefore, we cytometrically studied the mechanism of Zn2+-dependent lethal actions of Ziram on rat lymphocytes under environmentally relevant Zn2+ levels. Membrane and cellular parameters of rat lymphocytes were estimated by flow-cytometric techniques with appropriate fluorescent probes. The Ziram-induced increase in cell lethality was completely attenuated by Zn2+ chelators. A significant raise of cell lethality was found on the simultaneous application of Ziram at a sublethal concentration and ZnCl2. The combination of Ziram and ZnCl2 increased the cellular superoxide anion content and decreased the cellular GSH content, which possibly caused the increase in cell lethality. The zinc concentrations under present experimental conditions were comparable to the environmentally relevant concentrations found in rivers. Therefore, the environmental level of zinc may be critical in estimating the toxicity of Ziram to wild animals.
Journal Title
Toxicology Research
ISSN
20454538
Publisher
The Royal Society of Chemistry
Volume
6
Issue
4
Start Page
499
End Page
504
Published Date
2017-04-18
Rights
©2017 The Royal Society of Chemistry
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Bioscience and Bioindustry