ID 111989
Author
Kishida, Takumi Tokushima University
Akiyoshi, Kenji Tokushima University
Erdenedalai, Erdenebat Tokushima University
Enhetomuru, Anu Tokushima University
Keywords
Dibromoacetonitrile
Water disinfection
Nonprotein thiol
Intracellular Zn2+ level
Lymphocytes
Content Type
Journal Article
Description
The aim of this study was to investigate the effects of dibromoacetonitrile (DBAN), a by-product in water bacterial control, at sublethal concentrations on rat thymocytes, by using a cytometric technique with appropriate fluorescent dyes. By using this method, the possibility that DBAN induces cellular actions related to oxidative stress was assessed. DBAN reduced the content of cellular nonprotein thiols under Zn2+-free conditions. It elevated the intracellular level of Zn2+, being independent from external Zn2+. DBAN increased cell vulnerability to the cytotoxic action of hydrogen peroxide. These actions of DBAN were likely related to oxidative stress. DBAN is formed by the reaction of bromides and chlorinated oxidants during water disinfection. Hydrolysis of 2,2-dibromo-3-nitrilopropionamide, an antimicrobial used in hydraulic fracturing fluids for production of shale gas and oil, produces DBAN. Therefore, the concern regarding the levels of DBAN in industrial water systems is necessary to avoid the environmental risk to humans and wild mammals.
Journal Title
Toxicology in Vitro
ISSN
08872333
NCID
AA10678594
AA11540107
Publisher
Elsevier
Volume
51
Start Page
145
End Page
149
Published Date
2018-05-23
Rights
© 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Science and Technology
Bioscience and Bioindustry