ID 112035
Title Alternative
膵β細胞と膵α細胞の分化表現型はホメオティック遺伝子発現の差異と関連する
Author
Mizusawa, Noriko Department of Pharmacology, School of Dentistry, The University of Tokushima|Division of Genetic Information, Institute for Genome Research, The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Hasegawa, Tomoko The University of Tokushima
Harada, Nagakatsu The University of Tokushima KAKEN Search Researchers
Keywords
Pancreatic islet
β-cell
α-cell
Homeotic gene
Hox gene
Microarray
Content Type
Thesis or Dissertation
Description
To identify the genes that determine differentiation phenotypes, we compared gene expression of pancreatic islet β- and α-cells, which are derived from the common precursor and secrete insulin and glucagon, respectively. The expression levels of homeotic genes including Hox genes known to determine region specificity in the antero-posterior (AP) body axis, tissue-specific homeobox genes, and other 8,734 genes were compared in a β- and α-cell line of MIN6 and αTC1.6. The expression of homeotic genes were surveyed with reverse transcription-polymerase chain reaction (RT-PCR) using degenerate primers corresponding to invariant amino acid sequences within the homeodomain and subsequently with specific primers. Expression of Hoxc6, Hoxc9, Hoxc10, Pdx1, Cdx2, Gbx2, Pax4, and Hlxb9 genes in MIN6 was higher than those in αTC1.6, while expression of Hoxa2, Hoxa3, Hoxa5, Hoxa6, Hoxa7, Hoxa9, Hoxa10, Hoxa13, Hoxb3, Hoxb5, Hoxb6, Hoxb13, Hoxb8, and Brain4 genes in αTC1.6 was higher than those in MIN6. Out of 8,734 mouse genes screened with high-density mouse cDNA microarrays for MIN6- and αTC1.6-derived cDNA, 58 and 25 genes were differentially over- and under-expressed in MIN6, respectively. GLUTag, which is derived from a large bowel tumor and expresses the proglucagon gene, showed a comparatively similar expression profile to that of αTC1.6 in both homeotic and other genes analyzed in cDNA microarray.
Our results are consistent with the interpretation that not only the tissue-specific homeotic genes, but also Hox genes are related to differentiation phenotypes of pancreatic β- and α-cells rather than their regional specification of the body in vertebrates.
Journal Title
Gene
ISSN
03781119
NCID
AA00654385
AA11529840
Publisher
Elsevier
Volume
331
Start Page
53
End Page
63
Published Date
2004-03-28
Remark
論文本文の公開
本論文は,著者Noriko Mizusawaの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第1611号
Diploma Number
甲医第794号
Granted Date
2005-03-25
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
Oral Sciences
Institute of Advanced Medical Sciences
University Hospital
Medical Sciences