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ID 112035
タイトル別表記
膵β細胞と膵α細胞の分化表現型はホメオティック遺伝子発現の差異と関連する
著者
水澤, 典子 Department of Pharmacology, School of Dentistry, The University of Tokushima|Division of Genetic Information, Institute for Genome Research, The University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
Hasegawa, Tomoko The University of Tokushima
小杉, 知里 The University of Tokushima KAKEN研究者をさがす
原田, 永勝 The University of Tokushima KAKEN研究者をさがす
キーワード
Pancreatic islet
β-cell
α-cell
Homeotic gene
Hox gene
Microarray
資料タイプ
学位論文
抄録
To identify the genes that determine differentiation phenotypes, we compared gene expression of pancreatic islet β- and α-cells, which are derived from the common precursor and secrete insulin and glucagon, respectively. The expression levels of homeotic genes including Hox genes known to determine region specificity in the antero-posterior (AP) body axis, tissue-specific homeobox genes, and other 8,734 genes were compared in a β- and α-cell line of MIN6 and αTC1.6. The expression of homeotic genes were surveyed with reverse transcription-polymerase chain reaction (RT-PCR) using degenerate primers corresponding to invariant amino acid sequences within the homeodomain and subsequently with specific primers. Expression of Hoxc6, Hoxc9, Hoxc10, Pdx1, Cdx2, Gbx2, Pax4, and Hlxb9 genes in MIN6 was higher than those in αTC1.6, while expression of Hoxa2, Hoxa3, Hoxa5, Hoxa6, Hoxa7, Hoxa9, Hoxa10, Hoxa13, Hoxb3, Hoxb5, Hoxb6, Hoxb13, Hoxb8, and Brain4 genes in αTC1.6 was higher than those in MIN6. Out of 8,734 mouse genes screened with high-density mouse cDNA microarrays for MIN6- and αTC1.6-derived cDNA, 58 and 25 genes were differentially over- and under-expressed in MIN6, respectively. GLUTag, which is derived from a large bowel tumor and expresses the proglucagon gene, showed a comparatively similar expression profile to that of αTC1.6 in both homeotic and other genes analyzed in cDNA microarray.
Our results are consistent with the interpretation that not only the tissue-specific homeotic genes, but also Hox genes are related to differentiation phenotypes of pancreatic β- and α-cells rather than their regional specification of the body in vertebrates.
掲載誌名
Gene
ISSN
03781119
cat書誌ID
AA00654385
AA11529840
出版者
Elsevier
331
開始ページ
53
終了ページ
63
発行日
2004-03-28
備考
論文本文の公開
本論文は,著者Noriko Mizusawaの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第1611号
学位記番号
甲医第794号
学位授与年月日
2005-03-25
学位名
博士(医学)
学位授与機関
徳島大学
部局
歯学系
先端酵素学研究所
病院
医学系