ID 112154
Title Alternative
遺伝子銃による生体への遺伝子導入
Gene gun approches for DNA vaccine and cytokine gene therapy in protozoan parasite infection
Author
Himeno, Kunisuke The University of Tokushima
Keywords
DNA vaccine
gene therapy
gene gun
cytokine gene
protozoan parasite
Content Type
Journal Article
Description
The particle-mediated method for gene delivery with a gene gun utilizes a shock wave to accelerate DNA-coated gold particles into target cells or tissues. This gene delivery method is effective in various somatic tissues in vitro and in vivo. We have, herein, applied this gene delivery system to DNA vaccine and cytokine gene therapy for protozoan parasite infections. We used cDNA encoding 47 kDa of Plasmodium falciparum serine repeat antigen (SERA) that is a vaccine candidate antigen and did SERA DNA immunization with mice using gene gun. Significant SERA-specific antibodies (Abs) were observed by SERA DNA immunization. Furthermore, these Ab responses were enhanced and regulated by coinoculation of cytokine expression plasmid. For other application, we examined the effects of in vivo IL-12 gene treatment on the course of infection with obligate protozoa, Trypanosoma cruzi. Transfer with IL-12 expression plasmid in vivo regulated systemic immune responses and furthermore this treatment controlled the progression of experimental trypanosomiasis. Therefore, this gene gun approach may be a useful for DNA vaccine and gene therapy in a wide spectrum of diseases other than the protozoan parasite infection.
Journal Title
Shikoku Acta Medica
ISSN
00373699
NCID
AN00102041
Publisher
徳島医学会
Volume
55
Issue
5
Start Page
180
End Page
185
Sort Key
180
Published Date
1999-10-25
FullText File
language
jpn
TextVersion
Publisher
departments
Medical Sciences