ID 112154
タイトル別表記
遺伝子銃による生体への遺伝子導入
Gene gun approches for DNA vaccine and cytokine gene therapy in protozoan parasite infection
著者
姫野, 國祐 徳島大学
キーワード
DNA vaccine
gene therapy
gene gun
cytokine gene
protozoan parasite
資料タイプ
学術雑誌論文
抄録
The particle-mediated method for gene delivery with a gene gun utilizes a shock wave to accelerate DNA-coated gold particles into target cells or tissues. This gene delivery method is effective in various somatic tissues in vitro and in vivo. We have, herein, applied this gene delivery system to DNA vaccine and cytokine gene therapy for protozoan parasite infections. We used cDNA encoding 47 kDa of Plasmodium falciparum serine repeat antigen (SERA) that is a vaccine candidate antigen and did SERA DNA immunization with mice using gene gun. Significant SERA-specific antibodies (Abs) were observed by SERA DNA immunization. Furthermore, these Ab responses were enhanced and regulated by coinoculation of cytokine expression plasmid. For other application, we examined the effects of in vivo IL-12 gene treatment on the course of infection with obligate protozoa, Trypanosoma cruzi. Transfer with IL-12 expression plasmid in vivo regulated systemic immune responses and furthermore this treatment controlled the progression of experimental trypanosomiasis. Therefore, this gene gun approach may be a useful for DNA vaccine and gene therapy in a wide spectrum of diseases other than the protozoan parasite infection.
掲載誌名
四国医学雑誌
ISSN
00373699
cat書誌ID
AN00102041
出版者
徳島医学会
55
5
開始ページ
180
終了ページ
185
並び順
180
発行日
1999-10-25
フルテキストファイル
言語
jpn
著者版フラグ
出版社版
部局
医学系