ID 112814
Title Alternative
Shikonin inhibits inflammatory cytokine production
Author
Shindo, Satoru Tokushima University
Keywords
shikonin
IL-6
IL-8
CCL20
NF-κB
periodontal ligament cells
Content Type
Journal Article
Description
Shikonin, which is derived from Lithospermum erythrorhizon, a herb used in traditional medicine, has long been considered to be a useful treatment for various diseases in traditional oriental medicine. Shikonin has recently been reported to have several pharmacological properties, e.g., it has anti-microbial, anti-tumor, and anti-inflammatory effects. The aim of this study was to examine whether shikonin is able to influence the production of interleukin (IL)-6, IL-8, and/or chemokine C-C motif ligand (CCL)20, which contribute to the pathogenesis of periodontal disease, in human periodontal ligament cells (HPDLC). The production levels of IL-6, IL-8, and CCL20 in HPDLC were determined using an ELISA. Western blot analysis was used to detect nuclear factor kappa B (NF-κB) pathway activation in HPDLC. Shikonin prevented IL-1β- or tumor necrosis factor (TNF)-α-mediated IL-6, IL-8, and CCL20 production in HPDLC. Moreover, we found that shikonin suppressed the phosphorylation and degradation of inhibitor of kappa B-alpha (IκB-α) in IL-1β- or TNF-α-stimulated HPDLC. These findings suggest that shikonin could have direct beneficial effects against periodontal disease by reducing IL-6, IL-8, and CCL20 production in periodontal lesions.
Journal Title
Inflammation
ISSN
03603997
15732576
NCID
AA00673765
AA12117940
Publisher
Springer
Volume
39
Issue
3
Start Page
1124
End Page
1129
Published Date
2016-04-12
Remark
This is a post-peer-review, pre-copyedit version of an article published in Inflammation.
The final authenticated version is available online at: https://doi.org/10.1007/s10753-016-0344-0
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Oral Sciences