Traceless synthesis of protein thioesters using enzyme-mediated hydrazinolysis and subsequent self-editing of cysteinyl prolyl sequence

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Use this link to cite this item : https://repo.lib.tokushima-u.ac.jp/113348

ID	113348
Author	Komiya, Chiaki Tokushima University Shigenaga, Akira Tokushima University KAKEN Search Researchers Tsukimoto, Jun Tokushima University Ueda, Masahiro Tokushima University Morisaki, Takuya Tokushima University Inokuma, Tsubasa Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers Itoh, Kohji Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers Otaka, Akira Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Content Type	Journal Article
Description	A traceless thioester-producing protocol featuring carboxypeptidase Y-mediated hydrazinolysis of cysteinyl prolyl leucine-tagged peptides has been developed. The hydrazinolysis followed by thioesterification affords cysteinyl prolyl thioesters. Self-editing of the tag and subsequent trans-thioesterification yields peptide thioesters. The developed protocol was successfully applied to conversion of recombinant proteins to thioesters.
Journal Title	Chemical Communications
ISSN	13597345 1364548X
NCID	AA11071130 AA12455105
Publisher	The Royal Society of Chemistry
Volume	55
Issue	49
Start Page	7029
End Page	7032
Published Date	2019-05-23
Rights	©The Royal Society of Chemistry 2019
EDB ID	352461
DOI (Published Version)	10.1039/C9CC03583D
URL ( Publisher's Version )	https://doi.org/10.1039/C9CC03583D
FullText File	chemcom_55_49_7029.pdf 1.61 MB
language	eng
TextVersion	Author
departments	Pharmaceutical Sciences