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ID 114186
Title Alternative
PPARγ Loss Leads to Reduced Fertility
Author
Cui, Yongzhi National Institutes of Health
Claudio, Estefania National Institutes of Health
Siebenlist, Ulrich K. National Institutes of Health
Gonzalez, Frank J. National Institutes of Health
Flaws, Jodi University of Maryland
Wagner, Kay-Uwe University of Nebraska Medical Center
Hennighausen, Lothar National Institutes of Health
Content Type
Journal Article
Description
The peroxisome proliferation-activated receptor gamma (PPARγ) is expressed in many cell types including mammary epithelium, ovary, macrophages, and B- and T-cells. PPARγ has an anti-proliferative effect in pre-adipocytes and mammary epithelial cells, and treatment with its ligands reduced the progression of carcinogen-induced mammary tumors in mice. Because PPARγ-null mice die in utero it has not been possible to study its role in development and tumorigenesis in vivo. To investigate whether PPARγ is required for the establishment and physiology of different cell types, a cell-specific deletion of the gene was carried out in mice using the Cre-loxP recombination system. We deleted the PPARγ gene in mammary epithelium using WAP-Cre transgenic mice and in epithelial cells, B- and T-cells, and ovary cells using MMTV-Cre mice. The presence of PPARγ was not required for functional development of the mammary gland during pregnancy and for the establishment of B- and T-cells. In addition, no increase in mammary tumors was observed. However, loss of the PPARγ gene in oocytes and granulosa cells resulted in impaired fertility. These mice have normal populations of follicles, they ovulate and develop corpora lutea. Although progesterone levels are decreased and implantation rates are reduced, the exact cause of the impaired fertility remains to be determined.
Journal Title
Journal of Biological Chemistry
ISSN
1083351X
NCID
AA1202441X
Publisher
The American Society for Biochemistry and Molecular Biology
Volume
277
Issue
20
Start Page
17830
End Page
17835
Published Date
2002-03-07
Remark
This research was originally published in the Journal of Biological Chemistry. Yongzhi Cui, Keiko Miyoshi, Estefania Claudio, Ulrich K. Siebenlist, Frank J. Gonzalez, Jodi Flaws, Kay-Uwe Wagner and Lothar Hennighausen. Loss of the Peroxisome Proliferation-activated Receptor gamma (PPARγ) Does Not Affect Mammary Development and Propensity for Tumor Formation but Leads to Reduced Fertility. J. Biol. Chem. 2002; 277(20):17830-17835.
Rights
© The American Society for Biochemistry and Molecular Biology
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language
eng
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departments
Oral Sciences