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ID 114634
Title Alternative
腸炎ビブリオ3型分泌装置エフェクタータンパク質VopQは、上皮細胞の糖代謝を変化させる
V. parahaemolyticus effector modulates host metabolism
Author
NGUYEN, QUOC ANH Tokushima University|National Institute of Nutrition
Hatayama, Sho Tokushima University
Tentaku, Aya Tokushima University
Kido, Junko Tokushima University
Huong Bui, Thi Mai National Institute of Nutrition
Keywords
metabolomics
host-pathogen interaction
Vibrio parahaemolyticus
T3SS effector
Content Type
Thesis or Dissertation
Description
Vibrio parahaemolyticus is a Gram-negative halophilic pathogen that frequently causing acute gastroenteritis and occasional wound infection. V. parahaemolyticus contains several virulent factors, including Type III secretion systems (T3SSs) and thermostable direct hemolysin (TDH). In particular, T3SS1 is a potent cytotoxic inducer, and T3SS2 is essential for causing acute gastroenteritis. Although much is known about V. parahaemolyticus’s effector manipulating host signaling transductions, little is known about the host metabolomic changes modulated by V. parahaemolyticus. To address this knowledge gap, we performed a metabolomic analysis of the epithelial cells during V. parahaemolyticus infection using capillary electrophoresis–time-of-flight mass spectrometry (CE-TOF/MS). Our results revealed significant metabolomic perturbations upon V. parahaemolyticus infection. Moreover, we identified that T3SS1’s VopQ effector was responsible for inducing the significant metabolic changes in the infected cells. The VopQ effector dramatically altered the host cell’s glycolytic, tricarboxylic acid cycle (TCA), amino acid metabolisms. VopQ effector disrupted host cell redox homeostasis by depleting cellular glutathione and subsequently increasing the level of reactive oxygen species (ROS) production.
Journal Title
mSphere
ISSN
23795042
Publisher
American Society for Microbiology
Volume
5
Issue
2
Start Page
e00960-19
Published Date
2020-03-18
Remark
内容要旨・審査要旨・論文本文の公開
内容要旨・審査要旨:k3413_abstract_review.pdf
論文本文:k3413_fulltext.pdf (著者最終稿)
論文本文:msphere_5_2_e00960-19.pdf (雑誌掲載版)
本論文は, 著者Nguyen Quoc Anhの学位論文として提出され, 学位審査・授与の対象となっている。
Rights
© 2020 Nguyen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.( https://creativecommons.org/licenses/by/4.0/ )
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3413号
Diploma Number
甲栄第274号
Granted Date
2020-03-12
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences