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ID 114634
タイトル別表記
腸炎ビブリオ3型分泌装置エフェクタータンパク質VopQは、上皮細胞の糖代謝を変化させる
V. parahaemolyticus effector modulates host metabolism
著者
グェン, クォ アイン 徳島大学大学院栄養生命科学教育部(人間栄養科学専攻)
Hatayama, Sho Tokushima University
Tentaku, Aya Tokushima University
Kido, Junko Tokushima University
Huong Bui, Thi Mai National Institute of Nutrition
キーワード
metabolomics
host-pathogen interaction
Vibrio parahaemolyticus
T3SS effector
資料タイプ
学位論文
抄録
Vibrio parahaemolyticus is a Gram-negative halophilic pathogen that frequently causing acute gastroenteritis and occasional wound infection. V. parahaemolyticus contains several virulent factors, including Type III secretion systems (T3SSs) and thermostable direct hemolysin (TDH). In particular, T3SS1 is a potent cytotoxic inducer, and T3SS2 is essential for causing acute gastroenteritis. Although much is known about V. parahaemolyticus’s effector manipulating host signaling transductions, little is known about the host metabolomic changes modulated by V. parahaemolyticus. To address this knowledge gap, we performed a metabolomic analysis of the epithelial cells during V. parahaemolyticus infection using capillary electrophoresis–time-of-flight mass spectrometry (CE-TOF/MS). Our results revealed significant metabolomic perturbations upon V. parahaemolyticus infection. Moreover, we identified that T3SS1’s VopQ effector was responsible for inducing the significant metabolic changes in the infected cells. The VopQ effector dramatically altered the host cell’s glycolytic, tricarboxylic acid cycle (TCA), amino acid metabolisms. VopQ effector disrupted host cell redox homeostasis by depleting cellular glutathione and subsequently increasing the level of reactive oxygen species (ROS) production.
掲載誌名
mSphere
ISSN
23795042
出版者
American Society for Microbiology
5
2
開始ページ
e00960-19
発行日
2020-03-18
備考
内容要旨・審査要旨・論文本文の公開
内容要旨・審査要旨:k3413_abstract_review.pdf
論文本文:k3413_fulltext.pdf (著者最終稿)
論文本文:msphere_5_2_e00960-19.pdf (雑誌掲載版)
本論文は, 著者Nguyen Quoc Anhの学位論文として提出され, 学位審査・授与の対象となっている。
権利情報
© 2020 Nguyen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.( https://creativecommons.org/licenses/by/4.0/ )
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3413号
学位記番号
甲栄第274号
学位授与年月日
2020-03-12
学位名
博士(栄養学)
学位授与機関
徳島大学
部局
医学系