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ID 115195
Author
Hasi, Rumana Yesmin Tokushima University
Majima, Dai Tokushima University
Morito, Katsuya Tokushima University
Ali, Hanif Tokushima University
Nanjundan, Meera Tokushima University
Keywords
Sphingolipid
Phytoceramide 1-phosphate
Glycosylinositol phosphoceramide
TLC
Sephadex column chromatography
Plants
Content Type
Journal Article
Description
Glycosylinositol phosphoceramide (GIPC) is a sphingophospholipid in plants. Recently, we identified that GIPC is hydrolyzed to phytoceramide 1-phosphate (PC1P) by an uncharacterized phospholipase D activity following homogenization of certain plant tissues. We now developed methods for isolation of GIPC and PC1P from plant tissues and characterized their chemical stabilities. Hydrophilic solvents, namely a lower layer of a mixed solvent system consisting of isopropanol/hexane/water (55:20:25, v/v/v) was efficient solvent for extraction and eluent in column chromatography. GIPC was isolated by Sephadex column chromatography followed by TLC. A conventional method, such as the Bligh and Dyer method, was applicable for PC1P extraction. Specifically, PC1P was isolated by TLC following mild alkali treatment of lipid extracts of plants. The yields of GIPC and PC1P in our methods were both around 50-70%. We found that PC1P is tolerant against heat (up to 125 °C), strong acid (up to 10 M HCl), and mild alkali (0.1 M KOH). In contrast, significant degradation of GIPC occurred at 100 °C and 1.0 M HCl treatment, suggesting the instability of the inositol glycan moiety in these conditions. These data will be useful for further biochemical and nutritional studies on these sphingolipids.
Journal Title
Journal of Chromatography B
ISSN
15700232
NCID
AA11656178
AA11644805
Publisher
Elsevier
Volume
1152
Start Page
122213
Published Date
2020-06-12
Remark
論文本文は2022-06-12以降公開予定
Rights
© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
language
eng
TextVersion
その他
departments
Pharmaceutical Sciences
Bioscience and Bioindustry