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ID 115195
著者
Hasi, Rumana Yesmin Tokushima University
Majima, Dai Tokushima University
Morito, Katsuya Tokushima University
Ali, Hanif Tokushima University
Nanjundan, Meera Tokushima University
キーワード
Sphingolipid
Phytoceramide 1-phosphate
Glycosylinositol phosphoceramide
TLC
Sephadex column chromatography
Plants
資料タイプ
学術雑誌論文
抄録
Glycosylinositol phosphoceramide (GIPC) is a sphingophospholipid in plants. Recently, we identified that GIPC is hydrolyzed to phytoceramide 1-phosphate (PC1P) by an uncharacterized phospholipase D activity following homogenization of certain plant tissues. We now developed methods for isolation of GIPC and PC1P from plant tissues and characterized their chemical stabilities. Hydrophilic solvents, namely a lower layer of a mixed solvent system consisting of isopropanol/hexane/water (55:20:25, v/v/v) was efficient solvent for extraction and eluent in column chromatography. GIPC was isolated by Sephadex column chromatography followed by TLC. A conventional method, such as the Bligh and Dyer method, was applicable for PC1P extraction. Specifically, PC1P was isolated by TLC following mild alkali treatment of lipid extracts of plants. The yields of GIPC and PC1P in our methods were both around 50-70%. We found that PC1P is tolerant against heat (up to 125 °C), strong acid (up to 10 M HCl), and mild alkali (0.1 M KOH). In contrast, significant degradation of GIPC occurred at 100 °C and 1.0 M HCl treatment, suggesting the instability of the inositol glycan moiety in these conditions. These data will be useful for further biochemical and nutritional studies on these sphingolipids.
掲載誌名
Journal of Chromatography B
ISSN
15700232
cat書誌ID
AA11656178
AA11644805
出版者
Elsevier
1152
開始ページ
122213
発行日
2020-06-12
備考
論文本文は2022-06-12以降公開予定
権利情報
© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
出版社版DOI
出版社版URL
言語
eng
著者版フラグ
その他
部局
薬学系
生物資源系