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ID 116058
Title Alternative
マウス臼歯萌出後における線維芽細胞受容体 (Fgfr) 1, -2c, -3c 転写産物の発現
Author
Kano, Tsuyoshi Tokushima University
Keywords
Dentin formation
FGF receptor
Odontoblasts
Secondary dentin
Tooth eruption
Content Type
Thesis or Dissertation
Description
A previous study suggested that fibroblast growth factor (FGF) signaling plays an important role in dentin formation during tooth development. In this study, to examine dentin formation after tooth eruption involving secondary and tertiary dentin, we analyzed the expression patterns and expressing cells of Fgfr1, -2c, and -3c in mouse maxillary first molars (M1). Since it is difficult to recover the mRNAs from mineralized tissues, we tested methods for extraction after fixation and decalcification of teeth. We successfully obtained consistent results with quantitative real-time PCR (qPCR) using β-actin transcripts for validation. qPCR for Dentin sialo phosphoprotein (Dspp), Fgfr1, -2c, and -3c transcripts was performed on mice at ages of 2-20 weeks. The results showed that the highest expression levels of Dspp and Fgfr2c occurred at 2 weeks old followed by lower expression levels after 4 weeks old. However, the expression levels of Fgfr1 and Fgfr3c were constant throughout the experimental period. By in situ hybridization, Dspp, Fgfr1, and Fgfr3c transcripts were detected in odontoblasts at ages of 2 and 4 weeks. Also, Dspp and Fgfr1 transcripts were detected in odontoblasts facing reactionary dentin at 8 weeks old. These results suggest that FGF-FGFR signaling might be involved in the regulation of odontoblasts even after tooth eruption, including secondary and tertiary dentin formation. Moreover, our modified method for extracting mRNA from mineralized tissues after fixation and decalcification successfully produced consistent results.
Description Alternative
By utilizing our modified RNA extraction method, we determined the expression of FGFRs after tooth eruption, which suggested the commitment of FGF signaling to the homeostasis of odontoblasts.
Journal Title
Anatomical Science International
ISSN
14476959
1447073X
NCID
AA11619036
Publisher
Springer Nature|Japanese Association of Anatomists
Volume
96
Issue
2
Start Page
301
End Page
309
Published Date
2021-01-12
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Tsuyoshi Kanoの学位論文として提出され,学位審査・授与の対象となっている。
This is a post-peer-review, pre-copyedit version of an article published in Anatomical Science International. The final authenticated version is available online at: https://doi.org/10.1007/s12565-020-00594-4
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3492号
Diploma Number
甲口第470号
Granted Date
2021-03-23
Degree Name
Doctor of Philosophy
Grantor
Tokushima University
departments
Oral Sciences
University Hospital