ID | 116058 |
タイトル別表記 | マウス臼歯萌出後における線維芽細胞受容体 (Fgfr) 1, -2c, -3c 転写産物の発現
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著者 |
神尾, 強司
徳島大学大学院口腔科学教育部(口腔科学専攻)
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キーワード | Dentin formation
FGF receptor
Odontoblasts
Secondary dentin
Tooth eruption
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資料タイプ |
学位論文
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抄録 | A previous study suggested that fibroblast growth factor (FGF) signaling plays an important role in dentin formation during tooth development. In this study, to examine dentin formation after tooth eruption involving secondary and tertiary dentin, we analyzed the expression patterns and expressing cells of Fgfr1, -2c, and -3c in mouse maxillary first molars (M1). Since it is difficult to recover the mRNAs from mineralized tissues, we tested methods for extraction after fixation and decalcification of teeth. We successfully obtained consistent results with quantitative real-time PCR (qPCR) using β-actin transcripts for validation. qPCR for Dentin sialo phosphoprotein (Dspp), Fgfr1, -2c, and -3c transcripts was performed on mice at ages of 2-20 weeks. The results showed that the highest expression levels of Dspp and Fgfr2c occurred at 2 weeks old followed by lower expression levels after 4 weeks old. However, the expression levels of Fgfr1 and Fgfr3c were constant throughout the experimental period. By in situ hybridization, Dspp, Fgfr1, and Fgfr3c transcripts were detected in odontoblasts at ages of 2 and 4 weeks. Also, Dspp and Fgfr1 transcripts were detected in odontoblasts facing reactionary dentin at 8 weeks old. These results suggest that FGF-FGFR signaling might be involved in the regulation of odontoblasts even after tooth eruption, including secondary and tertiary dentin formation. Moreover, our modified method for extracting mRNA from mineralized tissues after fixation and decalcification successfully produced consistent results.
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抄録別表記 | By utilizing our modified RNA extraction method, we determined the expression of FGFRs after tooth eruption, which suggested the commitment of FGF signaling to the homeostasis of odontoblasts.
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掲載誌名 |
Anatomical Science International
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ISSN | 14476959
1447073X
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cat書誌ID | AA11619036
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出版者 | Springer Nature|Japanese Association of Anatomists
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巻 | 96
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号 | 2
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開始ページ | 301
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終了ページ | 309
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発行日 | 2021-01-12
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備考 | 内容要旨・審査要旨・論文本文の公開
本論文は,著者Tsuyoshi Kanoの学位論文として提出され,学位審査・授与の対象となっている。 This is a post-peer-review, pre-copyedit version of an article published in Anatomical Science International. The final authenticated version is available online at: https://doi.org/10.1007/s12565-020-00594-4 |
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出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
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著者版フラグ |
博士論文全文を含む
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文科省報告番号 | 甲第3492号
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学位記番号 | 甲口第470号
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学位授与年月日 | 2021-03-23
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学位名 |
博士(学術)
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学位授与機関 |
徳島大学
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部局 |
歯学系
病院
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