ID 23732
Author
Asahi, Yoshihiko Division of Molecular Genetics, Institute for Enzyme Research, The University of Tokushima
Hayashi, Hideki Division of Molecular Genetics, Institute for Enzyme Research, The University of Tokushima
Lihong, Wang Division of Molecular Genetics, Institute for Enzyme Research, The University of Tokushima
Ebina, Yousuke Division of Molecular Genetics, Institute for Enzyme Research, The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
GLUT4myc translocation
actin rearrangement
fluorescence microscope
Content Type
Journal Article
Description
We earlier developed a novel method to detect translocation of glucose transporter type 4 (GLUT4) directly, quantitatively and simply using c-MYC epitope-tagged GLUT4 (GLUT4myc) (Kanai F, Nishioka Y, Hayashi H, Kamohara S, Todaka M, Ebina Y:J Biol Chem 268:14523-14526, 1993). We further developed the method to visualize GLUT4myc on the cell surfac by fluorescence microscope using a highly sensitive immunochemical detection system in tissue culture cells stably expressing GLUT4myc. The translocation of GLUT4myc was observed on stimulation with insulin in 3T3-L1 adipocytes, CHO cells and L6 myotubes stably expressing GLUT4myc. Platelet-derived growth factor (PDGF), norepinephrine and bradykinin also triggered GLUT4 translocation in CHO-GLUT4myc cells stably expressing each receptor. To observe the distribution of GLUT4 and actin after insulin treatment, double staining for GLUT4myc and actin was performed. Translocated GLUT4myc on the cell surface was co-localized with rearranged actin in CHO cells and L6 myotubes. This result suggests that a correlation exists between GLUT4 translocation and actin rearrangement.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
46
Issue
3-4
Start Page
192
End Page
199
Sort Key
192
Published Date
1999
EDB ID
FullText File
language
eng
departments
Institute of Advanced Medical Sciences