ID 23732
著者
アサヒ, ヨシヒコ Division of Molecular Genetics, Institute for Enzyme Research, The University of Tokushima
ハヤシ, ヒデキ Division of Molecular Genetics, Institute for Enzyme Research, The University of Tokushima
Lihong, Wang Division of Molecular Genetics, Institute for Enzyme Research, The University of Tokushima
蛯名, 洋介 Division of Molecular Genetics, Institute for Enzyme Research, The University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
GLUT4myc translocation
actin rearrangement
fluorescence microscope
資料タイプ
学術雑誌論文
抄録
We earlier developed a novel method to detect translocation of glucose transporter type 4 (GLUT4) directly, quantitatively and simply using c-MYC epitope-tagged GLUT4 (GLUT4myc) (Kanai F, Nishioka Y, Hayashi H, Kamohara S, Todaka M, Ebina Y:J Biol Chem 268:14523-14526, 1993). We further developed the method to visualize GLUT4myc on the cell surfac by fluorescence microscope using a highly sensitive immunochemical detection system in tissue culture cells stably expressing GLUT4myc. The translocation of GLUT4myc was observed on stimulation with insulin in 3T3-L1 adipocytes, CHO cells and L6 myotubes stably expressing GLUT4myc. Platelet-derived growth factor (PDGF), norepinephrine and bradykinin also triggered GLUT4 translocation in CHO-GLUT4myc cells stably expressing each receptor. To observe the distribution of GLUT4 and actin after insulin treatment, double staining for GLUT4myc and actin was performed. Translocated GLUT4myc on the cell surface was co-localized with rearranged actin in CHO cells and L6 myotubes. This result suggests that a correlation exists between GLUT4 translocation and actin rearrangement.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
46
3-4
開始ページ
192
終了ページ
199
並び順
192
発行日
1999
EDB ID
66798
フルテキストファイル
言語
eng
部局
先端酵素学研究所