ID 286
Title Alternative
Multidrug Resistance and Apoptosis in Oral Squamous Carcinoma Cells : Role of Transcription Factors
Author
Okamura, Hirohiko Department of Histology and Oral Histology, Institute of Health Biosciences, The University of Tokushima Graduate School KAKEN Search Researchers
Keywords
口腔扁平上皮癌細胞
薬剤耐性
アポトーシス
転写調節因子
Content Type
Departmental Bulletin Paper
Description
Transcription factors are key factors of a number of cellular processes, such as cell growth, carcinogenesis, and apoptosis. Tumor cells including squamous carcinoma cells initially respond to chemotherapeutic drugs, however, they often acquire anti-apoptotic property and drug resistance. Human oral squamous carcinoma cell line SCCTF cells were isolated as minimal sensitivity to a variety of anticancer drugs, whereas SCCKN cells were isolated as highly sensitive to these reagents. We have clarified the molecular mechanisms that regulate apoptosis and the multidrug resistance using these cell lines. SCCTF cells expressed some transcription factors including EGR-1, NF-Y, and NF-kB. Okadaic acid and calyculin A, inhibitors of protein phosphatases type 1 and 2A, decreased EGR-1 expression and induced apoptosis in SCCKN cells. In contrast, these inhibitors stimulated the phosphorylation of EGR-1 protein in SCCTF cells during apoptosis. Activation of EGR-1 was involved in the up-regulation of PTEN, which in turn decreased phosphorylation level of AKT in SCCTF cells. Moreover, multidrug resistance-1 (MDR1) gene was expressed higher level in SCCTF cells. Inhibition of MDR1 promoted anticancer drug-induced apoptosis. NF-Y and its binding sites were demonstrated to play an important role in transcriptional regulation of MDR1 in SCCTF cells. In this review, we focus on the roles of transcription factors in MDR1 expression and apoptosis in oral squamous carcinoma cells through our recent studies.
Journal Title
四国歯学会雑誌
ISSN
09146091
NCID
AN10050046
Volume
19
Issue
2
Start Page
171
End Page
176
Sort Key
171
Published Date
2007-01
Remark
公開日:2010年1月24日で登録したコンテンツは、国立情報学研究所において電子化したものです。
EDB ID
FullText File
language
jpn
departments
Oral Sciences
Report Type
受賞講演