ID 286
タイトル別表記
Multidrug Resistance and Apoptosis in Oral Squamous Carcinoma Cells : Role of Transcription Factors
著者
岡村, 裕彦 徳島大学大学院ヘルスバイオサイエンス研究部口腔組織学分野 KAKEN研究者をさがす
キーワード
口腔扁平上皮癌細胞
薬剤耐性
アポトーシス
転写調節因子
資料タイプ
紀要論文
抄録
Transcription factors are key factors of a number of cellular processes, such as cell growth, carcinogenesis, and apoptosis. Tumor cells including squamous carcinoma cells initially respond to chemotherapeutic drugs, however, they often acquire anti-apoptotic property and drug resistance. Human oral squamous carcinoma cell line SCCTF cells were isolated as minimal sensitivity to a variety of anticancer drugs, whereas SCCKN cells were isolated as highly sensitive to these reagents. We have clarified the molecular mechanisms that regulate apoptosis and the multidrug resistance using these cell lines. SCCTF cells expressed some transcription factors including EGR-1, NF-Y, and NF-kB. Okadaic acid and calyculin A, inhibitors of protein phosphatases type 1 and 2A, decreased EGR-1 expression and induced apoptosis in SCCKN cells. In contrast, these inhibitors stimulated the phosphorylation of EGR-1 protein in SCCTF cells during apoptosis. Activation of EGR-1 was involved in the up-regulation of PTEN, which in turn decreased phosphorylation level of AKT in SCCTF cells. Moreover, multidrug resistance-1 (MDR1) gene was expressed higher level in SCCTF cells. Inhibition of MDR1 promoted anticancer drug-induced apoptosis. NF-Y and its binding sites were demonstrated to play an important role in transcriptional regulation of MDR1 in SCCTF cells. In this review, we focus on the roles of transcription factors in MDR1 expression and apoptosis in oral squamous carcinoma cells through our recent studies.
掲載誌名
四国歯学会雑誌
ISSN
09146091
cat書誌ID
AN10050046
19
2
開始ページ
171
終了ページ
176
並び順
171
発行日
2007-01
備考
公開日:2010年1月24日で登録したコンテンツは、国立情報学研究所において電子化したものです。
EDB ID
155301
フルテキストファイル
言語
jpn
部局
歯学系
記事種別
受賞講演