ID 74502
Title Alternative
低酸素細胞特異的な蛍光イメージング剤の開発
Author
Nakata, Eiji Division of Bioinformatics Engineering, Department of Life System, Institute of Technology and Science, Graduate School of the University of Tokushima
Yukimachi, Yoshihiro Graduate School of Advanced Technology and Science, The University of Tokushima
Abe, Chiaki Graduate School of Advanced Technology and Science, The University of Tokushima
Uto, Yoshihiro Division of Bioinformatics Engineering, Department of Life System, Institute of Technology and Science, Graduate School of the University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Hori, Hitoshi Division of Bioinformatics Engineering, Department of Life System, Institute of Technology and Science, Graduate School of the University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
Fluorescent pH probe
Tumor hypoxia
Fluorescent imaging
Bioreductive activation
Nitro reduction
Content Type
Departmental Bulletin Paper
Description
We have designed and evaluated UTX-12 as a novel fluorescent pH probe for tumor hypoxia
imaging. UTX-12 consists of a p-nitro benzyl moiety, which is a latent hypoxia-selective leaving
group activated by nitro reduction, directly linked to SNARF. Although UTX-12 itself is colorless and
non-fluorescent in aqueous solution, nitro reduction triggers the release of SNARF which has
well-characterized long wavelength absorption and fluorescence that is sensitive to pH. The
resultant SNARF, released intracellularly by enzymatic reduction of UTX-12, allows measurement of
pH by pH-dependent dual emission shifts. UTX-12 showed clear differences in fluorescence
behavior between hypoxic and aerobic conditions in liver microsomes and inside V79 cells. These
data are confirmation that UTX-12 is biologically reduced inside tumor cells and the released SNARF
should monitor intracellular pH of tumor cells selectively with reduced background signal.
Journal Title
徳島大学大学院ソシオテクノサイエンス研究部研究報告
ISSN
21859094
NCID
AA12214889
Volume
55
Published Date
2010
FullText File
language
eng
departments
Bioscience and Bioindustry