ID | 74502 |
タイトル別表記 | 低酸素細胞特異的な蛍光イメージング剤の開発
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著者 |
中田, 栄司
Division of Bioinformatics Engineering, Department of Life System, Institute of Technology and Science, Graduate School of the University of Tokushima
行待, 芳浩
Graduate School of Advanced Technology and Science, The University of Tokushima
阿部, 千秋
Graduate School of Advanced Technology and Science, The University of Tokushima
宇都, 義浩
Division of Bioinformatics Engineering, Department of Life System, Institute of Technology and Science, Graduate School of the University of Tokushima
徳島大学 教育研究者総覧
KAKEN研究者をさがす
堀, 均
Division of Bioinformatics Engineering, Department of Life System, Institute of Technology and Science, Graduate School of the University of Tokushima
徳島大学 教育研究者総覧
KAKEN研究者をさがす
|
キーワード | Fluorescent pH probe
Tumor hypoxia
Fluorescent imaging
Bioreductive activation
Nitro reduction
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資料タイプ |
紀要論文
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抄録 | We have designed and evaluated UTX-12 as a novel fluorescent pH probe for tumor hypoxia
imaging. UTX-12 consists of a p-nitro benzyl moiety, which is a latent hypoxia-selective leaving group activated by nitro reduction, directly linked to SNARF. Although UTX-12 itself is colorless and non-fluorescent in aqueous solution, nitro reduction triggers the release of SNARF which has well-characterized long wavelength absorption and fluorescence that is sensitive to pH. The resultant SNARF, released intracellularly by enzymatic reduction of UTX-12, allows measurement of pH by pH-dependent dual emission shifts. UTX-12 showed clear differences in fluorescence behavior between hypoxic and aerobic conditions in liver microsomes and inside V79 cells. These data are confirmation that UTX-12 is biologically reduced inside tumor cells and the released SNARF should monitor intracellular pH of tumor cells selectively with reduced background signal. |
掲載誌名 |
徳島大学大学院ソシオテクノサイエンス研究部研究報告
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ISSN | 21859094
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cat書誌ID | AA12214889
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巻 | 55
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発行日 | 2010
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フルテキストファイル | |
言語 |
eng
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部局 |
生物資源系
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