ID 74539
Title Transcription
チュウトウド コウネツセイ サイキン Geobacillus stearothermophilus セイイク オンド ヘンイカブ ニ オケル タンパクシツ ハツゲン カイセキ
Title Alternative
Protein Expression Analysis in Temparature-adaptated Mutant Strains of Moderately thermophile Geobacillus stearothermophilus
Author
Kyogoku, Hitomi Laboratory of Biochemistry, Department of Mathematical and Natural Sciences, Faculty of Integrated Arts and Sciences, The University of Tokushima
Yamaguchi, Yasuaki Laboratory of Biochemistry, Department of Mathematical and Natural Sciences, Faculty of Integrated Arts and Sciences, The University of Tokushima
Miyano-Ono, Momoyo Laboratory of Biochemistry, Department of Mathematical and Natural Sciences, Faculty of Integrated Arts and Sciences, The University of Tokushima
Yamaguchi, Ayano Laboratory of Biochemistry, Department of Mathematical and Natural Sciences, Faculty of Integrated Arts and Sciences, The University of Tokushima
Satoh, Takanori Laboratory of Biochemistry, Department of Mathematical and Natural Sciences, Faculty of Integrated Arts and Sciences, The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
Thermophile
Adaptation
Expression
Growth temperature
Geobacillus stearothermophilus
Content Type
Departmental Bulletin Paper
Description
Optimum growth of moderately thermophile Geobacillus stearothermophilus (Bst) has been observed at 55ºC, and suppressed by the downshift to lower temperature. In order to understand the relationship between growth temperature and protein expression, we made Bst mutant strains which were adapted to lower temperature (30ºC) compared with wild type strain, and then we examined their protein expression under various growth temperatures. When they were cultured at 55ºC, different expression patterns of six proteins (P1-P6) were observed between wild type and mutant strains, whereas five proteins (A1-A5) were found only in mutant strains cultured at 37ºC. Furthermore, expression of five proteins (DS1-DS5) was increased according to the downshift of growth temperature to 37ºC. Among all 16 proteins, abundant three proteins (P1, DS3, and DS5) were electroblotted to PVDF membrane, followed by analyzed N-terminal amino acid sequences. As a result, it was suggested that P1 must be Surface-layer protein A (sbsA), whereas DS3 and DS5 were unknown proteins.
Journal Title
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
NCID
AN10065859
Volume
23
Issue
3
Start Page
82
End Page
90
Sort Key
82
Published Date
2009
EDB ID
FullText File
language
jpn
departments
Science and Technology