ID 74539
タイトルヨミ
チュウトウド コウネツセイ サイキン Geobacillus stearothermophilus セイイク オンド ヘンイカブ ニ オケル タンパクシツ ハツゲン カイセキ
タイトル別表記
Protein Expression Analysis in Temparature-adaptated Mutant Strains of Moderately thermophile Geobacillus stearothermophilus
著者
京極, 仁美 徳島大学総合科学部自然システム学科生物化学研究室
山口, 安亮 徳島大学総合科学部自然システム学科生物化学研究室
小野(宮野), 百代 徳島大学総合科学部自然システム学科生物化学研究室
山口, 綾野 徳島大学総合科学部自然システム学科生物化学研究室
佐藤, 高則 徳島大学総合科学部自然システム学科生物化学研究室 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
Thermophile
Adaptation
Expression
Growth temperature
Geobacillus stearothermophilus
資料タイプ
紀要論文
抄録
Optimum growth of moderately thermophile Geobacillus stearothermophilus (Bst) has been observed at 55ºC, and suppressed by the downshift to lower temperature. In order to understand the relationship between growth temperature and protein expression, we made Bst mutant strains which were adapted to lower temperature (30ºC) compared with wild type strain, and then we examined their protein expression under various growth temperatures. When they were cultured at 55ºC, different expression patterns of six proteins (P1-P6) were observed between wild type and mutant strains, whereas five proteins (A1-A5) were found only in mutant strains cultured at 37ºC. Furthermore, expression of five proteins (DS1-DS5) was increased according to the downshift of growth temperature to 37ºC. Among all 16 proteins, abundant three proteins (P1, DS3, and DS5) were electroblotted to PVDF membrane, followed by analyzed N-terminal amino acid sequences. As a result, it was suggested that P1 must be Surface-layer protein A (sbsA), whereas DS3 and DS5 were unknown proteins.
掲載誌名
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
cat書誌ID
AN10065859
23
3
開始ページ
82
終了ページ
90
並び順
82
発行日
2009
EDB ID
194636
フルテキストファイル
言語
jpn
部局
理工学系