ID 74628
Author
Kimura, Kaori Faculty of Dentistry, The University of Tokushima
Nishimura, Yumiko Faculty of Dentistry, Okayama University
Oyama, Keisuke Faculty of Medicine, Saga University
Kawanai, Takuya Faculty of Integrated Arts and Sciences, The University of Tokushima
Hashimoto, Erika Faculty of Integrated Arts and Sciences, The University of Tokushima
Oyama, Yasuo Faculty of Integrated Arts and Sciences, The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
Tetracaine
Lidocaine
Zinc
Cytotoxicity
Content Type
Departmental Bulletin Paper
Description
In this study to examine the cytotoxic property of tetracaine, we cytometrically examined the effect of tetracaine on intracellular Zn2+ concentration by the use of FluoZin-3, a fluorescent indicator of intracellular Zn2+. Lidocaine was used as a reference drug. The incubation of rat thymocytes with tetracaine decreased the intensity of FluoZin-3 fluorescence while that with lidocaine increased the intensity. The incubation with 10 μM DTPA, a chelator for extracellular Zn2+, attenuated the tetracaine-induced decrease in fluorescence intensity. The application of ZnCl2 augmented FluoZin-3 fluorescence. The augmentation by ZnCl2 was a temperature-sensitive. Tetracaine attenuated the ZnCl2-induced augmentation of FluoZin-3 fluorescence. Taken together, the results suggest that tetracaine attenuates membrane Zn2+ influx, resulting in a decrease in intracellular Zn2+ concentration in rat thymocytes. Although the cells in this study are not targets for actions of local anesthetics, the result may give one clue to explain the difference between the cytotoxicity of local anesthetics since the action of tetracaine on FluoZin-3 fluorescence was opposite to that of lidocaine.
Journal Title
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
NCID
AN10065859
Volume
25
Issue
2
Start Page
7
End Page
13
Sort Key
7
Published Date
2011
EDB ID
FullText File
language
eng
departments
Bioscience and Bioindustry