ID 80823
Author
Kiyokawa, Machiko Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
Matsuzaki, Toshiya Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Iwasa, Takeshi Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Ogata, Rie Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
Murakami, Masahiro Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
Kinouchi, Riyo Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
Yoshida, Shinobu Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
Kuwahara, Akira Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Yasui, Toshiyuki Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Irahara, Minoru Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
orexin A
luteinizing hormone
neuropeptide Y
gonadotropin-releasing hormone
Content Type
Journal Article
Description
Objectives : Reproductive functions are influenced by various feeding regulators.
Orexin, which is one of orexinergic peptides, suppresses the pulsatile secretion of
luteinizing hormone (LH) in bilaterally ovariectomized (OVX) rats. However, the mechanism
of this effect is still not clear. To investigate whether neuropeptide Y (NPY) is involved
in the orexin A-mediated suppression of pulsatile LH secretion, we evaluated the
effects of NPY antibody on the suppressive effect of orexin A. Methods : Orexin A was administered
intracerebroventricularly (icv) and NPY antibody (NPY-Ab) was injected before
icv administration of orexin A in OVX rats. Pulsatile LH secretion was analyzed by
measuring serum LH concentrations in the next 2 h in blood samples drawn at 6-min intervals
by radioimmunoassay. Results : Administration of orexin A significantly reduced
the mean LH concentration and LH pulse frequency. Co-administration of NPY antibody
with orexin A significantly restored the suppressive effect of orexin A on the mean LH
concentration and LH pulse frequency. Conclusion : NPY mediated the suppressive effect
of intracerebroventricularly injected orexin A on pulsatile LH secretion, suggesting that
hypothalamic orexin suppressed pulsatile gonadotropin-releasing hormone (GnRH) secretion
via NPY in the hypothalamus of female rats.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
58
Issue
1-2
Start Page
11
End Page
18
Sort Key
11
Published Date
2011-02
Remark
The journal of medical investigation : http://medical.med.tokushima-u.ac.jp/jmi/index.html
EDB ID
FullText File
language
eng
departments
Medical Sciences
University Hospital