ID 80825
Author
Kasai, Yuka Department of Obstetrics and Gynecology, Tokushima Prefectural Central Hospital
Maegawa, Masahiko Department of Obstetrics and Gynecology, Tokushima Prefectural Central Hospital
Yamamoto, Satoshi Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
Kamada, Masaharu Department of Obstetrics and Gynecology,Health Insurance Naruto Hospital
Yasui, Toshiyuki Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Uemura, Hirokazu Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kobayashi, Ayako Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
Kaneyama, Masayo Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
Tani, Anna Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
Matsui, Sumika Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN Search Researchers
Kuwahara, Akira Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Matsuzaki, Toshiya Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Furumoto, Hiroyuki Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
Irahara, Minoru Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
raloxifene
cytokine
whole blood assay
Content Type
Journal Article
Description
Purpose : The aims of this study were to determine the effects of raloxifene
therapy on production of cytokines and in vitro effects of raloxifene on production of
cytokines by whole blood cultures. Methods :We obtained samples of peripheral blood
from 6 postmenopausal women with osteopenia at baseline and after 3 and 6 months of
raloxifene therapy and 10 postmenopausal women who did not receive raloxifene therapy.
Whole blood from raloxifene-treated women was stimulated with lipopolysaccharide
(LPS) or phytohemeagglutinin (PHA). Whole blood from postmenopausal women who
were not treated with raloxifene was preincubated with raloxifene at concentrations
of 10-10-10-7 M and then stimulated with LPS or PHA. Concentrations of IL-1β, IL-4, IL-6,
IL-12p40, IL-12p70, TNF-α and IFN-γ in the supernatant were measured by respective
ELISAs. Results : In ex vivo cultures, raloxifene therapy inhibited LPS-stimulated production
of IL-1β, IL-6, IL-12p40, IL-12p70 and TNF-α, but not PHA-stimulated production
of IL-4 and IFN-γ. In in vitro cultures, raloxifene at a concentration (10-9 M) inhibited
LPS-stimulated production of IL-1β, IL-6 and IL-12p40 and PHA-stimulated production
of IFN-γ. Conclusions : Raloxifene therapy decreases the production of IL-1β,
IL-6, IL-12 and TNF-α but not that of IL-4 and IFN-γ, suggesting that modulation of
cytokines could play a role in the mechanisms of the osteoprotective effect of raloxifene.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
58
Issue
1-2
Start Page
110
End Page
117
Sort Key
110
Published Date
2011-02
Remark
The journal of medical investigation : http://medical.med.tokushima-u.ac.jp/jmi/index.html
EDB ID
FullText File
language
eng
departments
Medical Sciences
University Hospital