ID 83624
Author
Chen, Tzu-Hsiu Department of Nutrition, The University of Tokushima School of Medicine
Takeshita, Keiko Department of Faculty of Environmental and Symbiotic Science, Prefectural University of Kumamoto
Sakai, Kentaro Department of Nutrition, The University of Tokushima School of Medicine
Komatsu, Tatsushi Department of Nutrition, The University of Tokushima School of Medicine
Kishino, Yasuo Department of Nutrition, The University of Tokushima School of Medicine
Yamamoto, Shigeru Department of Nutrition, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory
Keywords
allergic rhinitis
protein
amino acid
mouse
Content Type
Journal Article
Description
We studied the effect of various amino acid mixtures on nasal allergy induced by the intranasal application of toluene diisocyanate (TDI) in mice. In Experiment 1 (Exp. 1), mice were fed a 25% casein, soy protein isolate (SPI), egg white protein (EW) or gluten diet. In Experiment 2 (Exp. 2), mice were fed a 25% amino acid mixture diets patterned after casein (AA-casein), SPI (AA-SPI), EW protein (AA-EW) or gluten (AA-gluten). In Experiment 3 (Exp. 3) we modified the glutamine/glutamic acid (Gln/Glu) concentrations in the amino acid mixtures. Mice were fed a 25% AA-SPI, low Gln/Glu AA-SPI (LG-AA-SPI), AA-EW or high Gln/Glu AA-EW (HG-AA-EW) diet. At the 5th week, mice were divided into sensitized (sen-) and non-sensitized (ns-) groups. The mice in sensitized groups were treated with two courses of intranasal application of toluene diisocyanate (TDI) in ethyl acetate for 5 consecutive days, separated by 9 days rest. The non-sensitized groups of mice were treated with a vehicle. Nine days after the second sensitization, all mice were provoked by TDI. Nasal responses and serum IgE concentration were studied. The findings of Exp. 1 showed that the sen-EW group exhibited a lower body weight gain, higher nasal symptom score and higher IgE concentration than the other sensitized groups. The findings of Exp. 2 showed that the sen-EW group had a lower body weight gain, higher nasal symptom score and higher IgE concentration than the other sensitized groups. In Exp. 3, the AA-EW group showed a higher total nasal score and IgE concentration than the HG-AA-EW group, however, the findings of LG-AA-SPI and AA-SPI were similar. These findings demonstrated that amino acid mixtures affect nasal allergy induced by the intranasal application of TDI in mice.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
47
Issue
3-4
Start Page
128
End Page
137
Sort Key
128
Published Date
2000
Remark
FullText File
language
eng
departments
Medical Sciences