ID 83624
著者
Chen, Tzu-Hsiu Department of Nutrition, The University of Tokushima School of Medicine
タケシタ, ケイコ Department of Faculty of Environmental and Symbiotic Science, Prefectural University of Kumamoto
サカイ, ケンタロウ Department of Nutrition, The University of Tokushima School of Medicine
コマツ, タツシ Department of Nutrition, The University of Tokushima School of Medicine
キシノ, ヤスオ Department of Nutrition, The University of Tokushima School of Medicine
山本, 茂 Department of Nutrition, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧
キーワード
allergic rhinitis
protein
amino acid
mouse
資料タイプ
学術雑誌論文
抄録
We studied the effect of various amino acid mixtures on nasal allergy induced by the intranasal application of toluene diisocyanate (TDI) in mice. In Experiment 1 (Exp. 1), mice were fed a 25% casein, soy protein isolate (SPI), egg white protein (EW) or gluten diet. In Experiment 2 (Exp. 2), mice were fed a 25% amino acid mixture diets patterned after casein (AA-casein), SPI (AA-SPI), EW protein (AA-EW) or gluten (AA-gluten). In Experiment 3 (Exp. 3) we modified the glutamine/glutamic acid (Gln/Glu) concentrations in the amino acid mixtures. Mice were fed a 25% AA-SPI, low Gln/Glu AA-SPI (LG-AA-SPI), AA-EW or high Gln/Glu AA-EW (HG-AA-EW) diet. At the 5th week, mice were divided into sensitized (sen-) and non-sensitized (ns-) groups. The mice in sensitized groups were treated with two courses of intranasal application of toluene diisocyanate (TDI) in ethyl acetate for 5 consecutive days, separated by 9 days rest. The non-sensitized groups of mice were treated with a vehicle. Nine days after the second sensitization, all mice were provoked by TDI. Nasal responses and serum IgE concentration were studied. The findings of Exp. 1 showed that the sen-EW group exhibited a lower body weight gain, higher nasal symptom score and higher IgE concentration than the other sensitized groups. The findings of Exp. 2 showed that the sen-EW group had a lower body weight gain, higher nasal symptom score and higher IgE concentration than the other sensitized groups. In Exp. 3, the AA-EW group showed a higher total nasal score and IgE concentration than the HG-AA-EW group, however, the findings of LG-AA-SPI and AA-SPI were similar. These findings demonstrated that amino acid mixtures affect nasal allergy induced by the intranasal application of TDI in mice.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
47
3-4
開始ページ
128
終了ページ
137
並び順
128
発行日
2000
備考
フルテキストファイル
言語
eng
部局
医学系