ID 83931
Author
Abe, Akiko Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Furumoto, Hiroyuki Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
Yoshida, Kanako Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kato, Takeshi Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Saijo, Yasuyo Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
Irahara, Minoru Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
fms-like tyrosine kinase 3 ligand
gene gun
dendritic cells
Content Type
Journal Article
Description
Aim : Particle-mediated transfection is known as an efficient method of nonviral
gene transfer. Flt3 ligand (FL) is a growth factor for hematopoietic progenitors ; it
promotes the growth of dendritic cells (DC). DCs are powerful antigen-presenting cells
(APCs) and show a remarkable capacity to stimulate antigen-specific T-cell responses.
In this study, we intended to investigate the suppressive effect on tumor growth by gene
gun-mediated transfer of FL in a murine model. Methods : C57BL/6J mice were injected
intradermally with MCA205 cells. DNA (pNGVL-hFLex)-coated gold particles were delivered
to themouse skin surrounding the target tumor. The expression of FL was determined
by RT-PCR. Analyses by immunohistochemistry and fluorescence-activated cell
sorter (FACS) revealed an increase in the number of DC after treatment with FL. Results :
Gene gun-mediated pNGVL-hFLex transfer significantly inhibited the growth of the
MCA205 tumor. FL transfer markedly increased the number of CD11c+ DCs in the tumor
tissue. Further, the FL-transfected mice exhibited a significantly higher number of CD80+
MHC-II cells. Conclusion : We successfully performed FL therapy using an in vivo gene
gun in order to effectively mobilize DCs in situ and induce suppressive immunity.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
58
Issue
1-2
Start Page
39
End Page
45
Sort Key
39
Published Date
2011-02
Remark
The journal of medical investigation : http://medical.med.tokushima-u.ac.jp/jmi/index.html
EDB ID
FullText File
language
eng
departments
Medical Sciences
University Hospital